Área de Fisiología Vegetal, Departamento de Ingeniería y Ciencias Agrarias, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, Campus Vegazana, s/n, E-24007 León, Spain.
Carbohydr Polym. 2022 Jan 15;276:118781. doi: 10.1016/j.carbpol.2021.118781. Epub 2021 Oct 18.
Plant cell walls provide essential functions in cell recognition, differentiation, adhesion and wound responses. Therefore, it is tempting to hypothesize that cell walls play a key role in grafting, but to date there are no quantitative studies targeting on cell wall changes during grafting. The aim of this work was to investigate the dynamics of pectic and hemicellulosic polysaccharides at the graft junctions in tomato homografts throughout the first 12 days after grafting. Cell wall fractionation, combined with ATR-FTIR spectroscopy and gas-chromatography, evidenced a marked increase in pectin content and a decrease in the degree of methyl-esterification of homogalacturonan in scion and rootstock throughout grafting. Also, recovery of tightly-bound hemicelluloses decreased at late times after grafting suggesting an increase of cross-linked hemicelluloses along grafting. In addition, immuno-dot assays revealed an increase in xyloglucan and arabinogalactan proteins in the first days after grafting, pointing to a presumed role in tissue adhesion-cohesion.
植物细胞壁在细胞识别、分化、黏附和创伤反应中提供重要功能。因此，人们很容易假设细胞壁在嫁接中起着关键作用，但迄今为止，还没有针对嫁接过程中细胞壁变化的定量研究。本工作旨在研究番茄同源嫁接后前 12 天接穗和砧木嫁接处果胶和半纤维素多糖的动态变化。细胞壁分级分离，结合衰减全反射傅里叶变换红外光谱和气相色谱，证明了在嫁接过程中，接穗和砧木中的果胶含量显著增加，同质半乳糖醛酸的甲酯化程度降低。此外，在嫁接后晚期，与细胞壁紧密结合的半纤维素的恢复减少，表明随着嫁接的进行，交联半纤维素增加。此外，免疫斑点检测显示，嫁接后最初几天木葡聚糖和阿拉伯半乳聚糖蛋白增加，这表明它们在组织黏附和黏合中可能发挥作用。
