Moon Suyun, Hur Onsook, Kim Seong-Hoon, Lee Yoonjung, Oh Hyeonseok, Yi Jungyoon, Ko Ho-Cheol, Woo Hee-Jong, Ro Nayoung, Na Young-Wang
National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of Korea.
Department of Crop Breeding, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of Korea.
Plants (Basel). 2024 Dec 19;13(24):3552. doi: 10.3390/plants13243552.
Lettuce () is a globally significant leafy vegetable, valued for both its economic and nutritional contributions. The efficient conservation and use of the lettuce germplasm are crucial for breeding and genetic improvement. This study examined the genetic diversity and population structure of a core collection of the lettuce germplasm using genotyping by sequencing (GBS). A total of 7136 high-quality single-nucleotide polymorphisms (SNPs) were identified across nine chromosomes. Population analysis through Bayesian clustering and discriminant analysis of principal components (DAPC) revealed three distinct genetic clusters. Cluster 2 exhibited the greatest genetic diversity ( = 0.29, = 0.44), while Cluster 3 had high levels of inbreeding ( = 0.79). Agro-morphological trait evaluation further identified significant differences in leaf length, plant weight, and head height across clusters. These findings provide valuable insights into the genetic and phenotypic diversity of lettuce, facilitating the development of more robust breeding programs. Additionally, the core collection established in this study offers a representative subset of the lettuce germplasm for future genomic research and conservation efforts.
生菜（）是一种在全球具有重要意义的叶菜类蔬菜，因其经济价值和营养贡献而受到重视。生菜种质资源的有效保存和利用对于育种和遗传改良至关重要。本研究采用简化基因组测序（GBS）技术，对生菜种质资源核心收集品的遗传多样性和群体结构进行了研究。在九条染色体上共鉴定出7136个高质量单核苷酸多态性（SNP）。通过贝叶斯聚类和主成分判别分析（DAPC）进行的群体分析揭示了三个不同的遗传簇。簇2表现出最大的遗传多样性（ = 0.29， = 0.44），而簇3具有较高的近交水平（ = 0.79）。农艺形态性状评价进一步确定了各簇在叶长、株重和头高方面存在显著差异。这些发现为生菜的遗传和表型多样性提供了有价值的见解，有助于制定更强大的育种计划。此外，本研究建立的核心收集品为生菜种质资源提供了一个具有代表性的子集，用于未来的基因组研究和保护工作。
