Russo Carla, Curcio Giuseppe, Graziani Alessandro, Mencacci Antonella, Pietrella Donatella
Medical Microbiology Unit, Department of Medicine and Surgery, University of Perugia, Piazzale Severi, Building D, 4th Floor, 06129 Perugia, Italy.
Microbiology Unit, Santa Maria Della Misericordia Hospital, 06129 Perugia, Italy.
Pharmaceutics. 2024 Dec 11;16(12):1580. doi: 10.3390/pharmaceutics16121580.
: Chronic skin wounds are characterized by inflammation, persistent infections, and tissue necrosis. The presence of bacterial biofilms prolongs the inflammatory response and delays healing. Ozone is a potent antimicrobial molecule, and many formulations have been used in the advanced therapeutic treatment of chronic wounds. The aim of this work was to determine the antimicrobial, anti-inflammatory, and regenerative activity of a stable ozone-gel formulation over time. : The antimicrobial property was assessed by measuring the minimal inhibitory concentration and the antibiofilm activity. The anti-inflammatory effect was evaluated by TNF-α determination, and the regenerative effect was measured by scratch assay. : The ozone gel demonstrated antimicrobial and antibiofilm activity in all ATCC microorganisms examined and on most clinical isolates. Higher concentrations of the ozone gel were also useful in the dispersion of preformed biofilm. The ozone gel also showed anti-inflammatory activity by reducing the production of TNF-α and regenerative activity in human fibroblasts and keratinocytes. : Given all these antimicrobial, anti-inflammatory, and regenerative characteristics, the ozone gel could be, in this formulation, used in the treatment of wounds. The ozone-gel formulation described here retains stability for over 30 months, which facilitates its use compared to formulations that lose efficacy quickly.
慢性皮肤伤口的特征为炎症、持续性感染和组织坏死。细菌生物膜的存在会延长炎症反应并延迟愈合。臭氧是一种强效抗菌分子，许多制剂已用于慢性伤口的先进治疗。这项工作的目的是确定一种稳定的臭氧凝胶制剂随时间的抗菌、抗炎和再生活性。通过测量最低抑菌浓度和抗生物膜活性来评估抗菌性能。通过测定肿瘤坏死因子-α（TNF-α）来评估抗炎作用，并通过划痕试验测量再生作用。臭氧凝胶在所检测的所有美国典型培养物保藏中心（ATCC）微生物以及大多数临床分离株中均表现出抗菌和抗生物膜活性。较高浓度的臭氧凝胶对已形成的生物膜的分散也有效。臭氧凝胶还通过减少TNF-α的产生表现出抗炎活性，并在人成纤维细胞和角质形成细胞中具有再生活性。鉴于所有这些抗菌、抗炎和再生特性，在此制剂中，臭氧凝胶可用于伤口治疗。此处描述的臭氧凝胶制剂可保持稳定性超过30个月，与那些很快失去效力的制剂相比，这便于其使用。
