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基于防污表面化学的声波传感器对卵巢癌生物标志物的检测

Acoustic Wave Sensor Detection of an Ovarian Cancer Biomarker with Antifouling Surface Chemistry.

作者信息

Davoudian Katharina, Spagnolo Sandro, Chan Edmund, Hianik Tibor, Thompson Michael

机构信息

Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.

Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovakia.

出版信息

Sensors (Basel). 2024 Dec 10;24(24):7884. doi: 10.3390/s24247884.

DOI:10.3390/s24247884
PMID:39771624
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11678973/
Abstract

Ovarian cancer (OC) must be detected in its early stages when the mortality rate is the lowest to provide patients with the best chance of survival. Lysophosphatidic acid (LPA) is a critical OC biomarker since its levels are elevated across all stages and increase with disease progression. This paper presents an LPA assay based on a thickness shear mode acoustic sensor with dissipation monitoring that involves a new thiol molecule 3-(2-mercaptoethanoxy)propanoic acid (HS-MEG-COOH). HS-MEG-COOH is an antifouling linker that provides (a) antifouling properties for gold substrates and (b) linking ability via its terminal carboxylic acid functional group. The antifouling ability of HS-MEG-COOH was tested in whole human serum. The new molecule was applied to the LPA assay in conjunction with a spacer molecule, 2-(2-mercaptoethoxy)ethan-1-ol (HS-MEG-OH), in a 1:1 / ratio. HS-MEG-COOH was covalently linked to gelsolin-actin, a protein complex probe that dissociates due to LPA-binding. LPA was detected in phosphate-buffered saline and undiluted human serum and achieved a low limit of detection (1.0 and 0.7 μM, respectively) which was below the concentration of LPA in healthy individuals. The antifouling properties of HS-MEG-COOH and the detection of LPA demonstrate the ability of the sensor to successfully identify the early-stage OC biomarker in undiluted human serum.

摘要

卵巢癌（OC）必须在死亡率最低的早期阶段被检测出来，以便为患者提供最佳的生存机会。溶血磷脂酸（LPA）是一种关键的OC生物标志物，因为其水平在所有阶段都会升高，并随着疾病进展而增加。本文介绍了一种基于厚度剪切模式声学传感器并带有耗散监测的LPA检测方法，该方法涉及一种新的硫醇分子3-(2-巯基乙氧基)丙酸（HS-MEG-COOH）。HS-MEG-COOH是一种防污连接剂，它为金基底提供（a）防污性能，以及（b）通过其末端羧酸官能团的连接能力。在全人血清中测试了HS-MEG-COOH的防污能力。这种新分子与间隔分子2-(2-巯基乙氧基)乙醇（HS-MEG-OH）以1:1的比例一起应用于LPA检测。HS-MEG-COOH与凝溶胶蛋白-肌动蛋白共价连接，凝溶胶蛋白-肌动蛋白是一种因LPA结合而解离的蛋白质复合探针。在磷酸盐缓冲盐水和未稀释的人血清中检测到了LPA，并且实现了较低的检测限（分别为1.0和0.7 μM），该检测限低于健康个体中LPA的浓度。HS-MEG-COOH的防污性能以及LPA的检测证明了该传感器能够在未稀释的人血清中成功识别早期OC生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/46c6a78df774/sensors-24-07884-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/5527d212880d/sensors-24-07884-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/cbb9716f0119/sensors-24-07884-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/f3269cf85b2e/sensors-24-07884-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/a96b960101e7/sensors-24-07884-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/a43541ffc608/sensors-24-07884-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/f1d98508480a/sensors-24-07884-sch004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/113763ef3c55/sensors-24-07884-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/316f39fbc85d/sensors-24-07884-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/98a3ddf25bf6/sensors-24-07884-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/1df8edc342af/sensors-24-07884-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/46c6a78df774/sensors-24-07884-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/5527d212880d/sensors-24-07884-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/cbb9716f0119/sensors-24-07884-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/f3269cf85b2e/sensors-24-07884-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/a96b960101e7/sensors-24-07884-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/a43541ffc608/sensors-24-07884-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/f1d98508480a/sensors-24-07884-sch004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/113763ef3c55/sensors-24-07884-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/316f39fbc85d/sensors-24-07884-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/98a3ddf25bf6/sensors-24-07884-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/1df8edc342af/sensors-24-07884-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f960/11678973/46c6a78df774/sensors-24-07884-g007.jpg

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Biomolecules. 2023 Sep 21;13(9):1426. doi: 10.3390/biom13091426.
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