Sookhoo Jamie R V, Schiffman Zachary, Ambagala Aruna, Kobasa Darwyn, Pardee Keith, Babiuk Shawn
Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, MB R3E 3R2, Canada.
Department of Immunology, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.
Vaccines (Basel). 2024 Nov 28;12(12):1344. doi: 10.3390/vaccines12121344.
Several protein expression platforms exist for a wide variety of biopharmaceutical needs. A substantial proportion of research and development into protein expression platforms and their optimization since the mid-1900s is a result of the production of viral antigens for use in subunit vaccine research. This review discusses the seven most popular forms of expression systems used in the past decade-bacterial, insect, mammalian, yeast, algal, plant and cell-free systems-in terms of advantages, uses and limitations for viral antigen production in the context of subunit vaccine research. Post-translational modifications, immunogenicity, efficacy, complexity, scalability and the cost of production are major points discussed. Examples of licenced and experimental vaccines are included along with images which summarize the processes involved.
针对各种各样的生物制药需求，存在多种蛋白质表达平台。自20世纪中叶以来，蛋白质表达平台及其优化的大量研发工作是出于生产用于亚单位疫苗研究的病毒抗原的目的。本综述讨论了过去十年中使用的七种最流行的表达系统——细菌、昆虫、哺乳动物、酵母、藻类、植物和无细胞系统——在亚单位疫苗研究背景下用于病毒抗原生产的优势、用途和局限性。翻译后修饰、免疫原性、功效、复杂性、可扩展性和生产成本是讨论的要点。文中包含了已获许可和实验性疫苗的例子以及总结所涉及过程的图片。
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