van Oers Monique M
Laboratory of Virology, Wageningen University, Binnenhaven 11 6709 PD, Wageningen, The Netherlands.
Adv Virus Res. 2006;68:193-253. doi: 10.1016/S0065-3527(06)68006-8.
The baculovirus-insect cell expression system is an approved system for the production of viral antigens with vaccine potential for humans and animals and has been used for production of subunit vaccines against parasitic diseases as well. Many candidate subunit vaccines have been expressed in this system and immunization commonly led to protective immunity against pathogen challenge. The first vaccines produced in insect cells for animal use are now on the market. This chapter deals with the tailoring of the baculovirus-insect cell expression system for vaccine production in terms of expression levels, integrity and immunogenicity of recombinant proteins, and baculovirus genome stability. Various expression strategies are discussed including chimeric, virus-like particles, baculovirus display of foreign antigens on budded virions or in occlusion bodies, and specialized baculovirus vectors with mammalian promoters that express the antigen in the immunized individual. A historical overview shows the wide variety of viral (glyco)proteins that have successfully been expressed in this system for vaccine purposes. The potential of this expression system for antiparasite vaccines is illustrated. The combination of subunit vaccines and marker tests, both based on antigens expressed in insect cells, provides a powerful tool to combat disease and to monitor infectious agents.
杆状病毒-昆虫细胞表达系统是一种经批准的用于生产具有人类和动物疫苗潜力的病毒抗原的系统，也已用于生产抗寄生虫病的亚单位疫苗。许多候选亚单位疫苗已在该系统中表达，免疫通常会产生针对病原体攻击的保护性免疫。首批在昆虫细胞中生产的兽用疫苗现已上市。本章从重组蛋白的表达水平、完整性和免疫原性以及杆状病毒基因组稳定性等方面探讨了杆状病毒-昆虫细胞表达系统在疫苗生产中的定制。讨论了各种表达策略，包括嵌合体、病毒样颗粒、杆状病毒在出芽病毒粒子或包涵体上展示外源抗原，以及带有哺乳动物启动子的专门杆状病毒载体，该载体在免疫个体中表达抗原。历史概述展示了为疫苗目的已在该系统中成功表达的多种病毒（糖）蛋白。阐述了该表达系统用于抗寄生虫疫苗的潜力。基于在昆虫细胞中表达的抗原的亚单位疫苗和标记检测的结合，为抗击疾病和监测感染因子提供了一个强大的工具。
