Wang Qikai, Zhang Ran, Wang Yue, Wang Ying, Liang Libin, Ma Haili, Wang Haidong, Si Longlong, Wu Xingchen
College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Viruses. 2024 Dec 23;16(12):1964. doi: 10.3390/v16121964.
Coinfections with porcine circovirus types 2, 3, and 4 (PCV2, PCV3, and PCV4) are increasingly being detected in the swine industry. However, there is no commercially available vaccine which prevents coinfection with PCV2, PCV3, and PCV4. The development of a vaccine expressing capsid (Cap) fusion proteins of multiple PCVs represents a promising approach for broadly preventing infection with PCVs. In this study, we developed a PCV subunit vaccine candidate (Cap 2-3-4) by predicting, screening, and fusing antigenic epitopes of Cap proteins of PCV2, PCV3, and PCV4. Immunoprotection assays showed that the prokaryotic expression of Cap 2-3-4 could effectively induce high levels of PCV2, PCV3, and PCV4 Cap-specific antibodies and successfully neutralize both PCV2 and PCV3. Furthermore, Cap 2-3-4 demonstrated a potent ability to activate cellular immunity and thus prevent lung damage in mice. This study provides a new option for the development of broad vaccines against PCVs.
猪圆环病毒2型、3型和4型（PCV2、PCV3和PCV4）的混合感染在养猪业中越来越多地被检测到。然而，目前尚无预防PCV2、PCV3和PCV4混合感染的商业化疫苗。开发一种表达多种猪圆环病毒衣壳（Cap）融合蛋白的疫苗是广泛预防猪圆环病毒感染的一种有前景的方法。在本研究中，我们通过预测、筛选和融合PCV2、PCV3和PCV4的Cap蛋白的抗原表位，开发了一种猪圆环病毒亚单位疫苗候选物（Cap 2-3-4）。免疫保护试验表明，Cap 2-3-4的原核表达能够有效诱导高水平的PCV2、PCV3和PCV4 Cap特异性抗体，并成功中和PCV2和PCV3。此外，Cap 2-3-4显示出激活细胞免疫并因此预防小鼠肺损伤的强大能力。本研究为开发针对猪圆环病毒的广谱疫苗提供了新的选择。
