Chen Guixian
Blood Purification Center, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, PR China.
J Biomater Appl. 2025 Apr;39(9):1084-1096. doi: 10.1177/08853282241304396. Epub 2025 Jan 7.
Acute kidney injury (AKI) resulting from cisplatin (Cs) chemotherapy presents a significant challenge in clinical management. The study aimed to fabricate a novel compound Polyvinylpyrrolidone-catechol-derived chitosan nanoconjugates (PCChi-NC) for targeting Cs-induced AKI. Characterization studies utilizing UV-visible spectrophotometry, FT-IR, XRD, and TEM revealed a spherical morphology with diameters ranging from 20 to 60 nm. In vitro assessments utilizing HEK 293 cell lines demonstrated the biocompatibility of PCChi-NC without eliciting toxic effects. Furthermore, PCChi-NC exhibited a notable reduction in Cs-induced cell death in kidney cells, as evidenced by biomarker analysis. Anti-inflammatory analysis of mouse kidney homogenates revealed a decrease in TNF-α and IL-1β levels, indicative of the therapeutic efficacy of PCChi-NC in mitigating Cs-induced kidney inflammation. Moreover, In vivo, experimental analysis was evidenced by stable body weight and histopathological changes in mice. Our findings highlight the potential of PCChi-NC as a promising candidate for targeted therapy in Cs-induced AKI, owing to its unique renal targeting capacity.
顺铂（Cs）化疗导致的急性肾损伤（AKI）在临床管理中是一项重大挑战。该研究旨在制备一种新型化合物聚乙烯吡咯烷酮 - 儿茶酚衍生的壳聚糖纳米共轭物（PCChi - NC）用于靶向Cs诱导的AKI。利用紫外可见分光光度法、傅里叶变换红外光谱、X射线衍射和透射电子显微镜进行的表征研究显示其呈球形形态，直径范围为20至60纳米。利用HEK 293细胞系进行的体外评估证明了PCChi - NC的生物相容性，且未引发毒性作用。此外，生物标志物分析表明，PCChi - NC在肾细胞中显著降低了Cs诱导的细胞死亡。对小鼠肾脏匀浆的抗炎分析显示肿瘤坏死因子 - α（TNF - α）和白细胞介素 - 1β（IL - 1β）水平降低，表明PCChi - NC在减轻Cs诱导的肾脏炎症方面具有治疗效果。此外，在体内，小鼠体重稳定和组织病理学变化证明了实验分析结果。我们的研究结果突出了PCChi - NC因其独特的肾脏靶向能力，作为Cs诱导的AKI靶向治疗有前景的候选物的潜力。
