一株五氯苯酚降解菌的分离与鉴定
Isolation and characterization of a pentachlorophenol-degrading bacterium.
作者信息
Stanlake G J, Finn R K
出版信息
Appl Environ Microbiol. 1982 Dec;44(6):1421-7. doi: 10.1128/aem.44.6.1421-1427.1982.
Abstract
With a new enrichment protocol, pentachlorophenol (PCP)-degrading bacteria were isolated from soil, water, and sewage. When characterized, all isolates were related and shared characteristics of the genus Arthrobacter. Growth rates for strain NC were determined for a number of substrates, including PCP and 2,4,6-trichlorophenol. Changes in PCP concentration affected growth rate and length of the lag phase but not cell yield. Increasing the pH from 6.8 to 7.8 decreased the length of the lag phase for growth on PCP. Cessation of growth, upon incremental addition of PCP, was found to be related to a decrease in pH rather than to a buildup of a toxic metabolite. Degradation of PCP by strain NC was shown to be complete.
摘要
采用一种新的富集培养方法，从土壤、水和污水中分离出了五氯苯酚（PCP）降解菌。经鉴定，所有分离菌株都具有相关性，且具有节杆菌属的特征。测定了菌株NC在多种底物上的生长速率，包括五氯苯酚和2,4,6 - 三氯苯酚。五氯苯酚浓度的变化影响生长速率和延滞期的长短，但不影响细胞产量。将pH从6.8提高到7.8可缩短菌株NC在五氯苯酚上生长的延滞期。发现随着五氯苯酚的逐步添加，生长停止与pH降低有关，而非与有毒代谢产物的积累有关。菌株NC对五氯苯酚的降解被证明是完全的。
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