胰腺腺癌中ARMCs的表达及预后价值的综合分析

Comprehensive analysis of the expression and prognostic value of ARMCs in pancreatic adenocarcinoma.

作者信息

Zhuo Guanxiang, Lin Shengzhai, Yuan Fei, Zheng Qiaoling, Guo Yinpin, Wang Zuwei, Hu Jianfei, Yao Meihong, Zhong Fuxiu, Chen Shi, Chen Yanling, Chen Huixing

机构信息

Department of Hepatobiliary Surgery, Fujian Institute of Hepatobiliary Surgery, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, China.

Department of Hepatobiliary Surgery, Fudan University Shanghai Cancer Center Xiamen Hospital, Xiamen, 350003, Fujian, China.

出版信息

BMC Cancer. 2025 Jan 7;25(1):28. doi: 10.1186/s12885-024-13365-5.

DOI:10.1186/s12885-024-13365-5

PMID:39773340

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11708071/

Abstract

BACKGROUND

Pancreatic adenocarcinoma (PAAD) has a very poor prognosis, and there are few treatments for PAAD. Therefore, it is important to find some biomarkers for the diagnosis and treatment of PAAD. Although some members of Armadillo repeat containing proteins (ARMCs) have been implicated in the development of certain cancers, their relationship with PAAD remains unknown. In this study, we aimed to explore the expression and prognostic value of ARMCs in PAAD.

METHODS

We used the The Cancer Genome Atlas (TCGA) database for survival analysis. Then, Gene Expression Profiling Interactive Analysis (GEPIA), the cBioPortal database, the Human Protein Atlas (HPA), Kaplan-Meier Plotter, LinkedOmics Database, Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG), Cytoscape and Timer were used to analyze the relationship between ARMCs and PAAD. In addition, we established a prognostic model of ARMCs for PAAD. Immunohistochemistry (IHC) was also performed. Then Image-J was used to analyze all images obtained from the experiment, and GraphPad-Prism (9.5.1) was used for statistical analysis to verify the expression of ARMCs in PAAD.

RESULTS

In the TCGA database, the expressions of ARMC1, 2, 3, 5, 6, 7, 8, 9 and 10 in PAAD tissues were significantly higher than those in normal tissues. And higher expressions of ARMC1 and 10 were associated with lower survival rate of PAAD patients. In addition, ARMC2, 5, 6, and 10 were positively associated with advanced stages of PAAD. ARMCs mutations occur in 11% of PAAD patients, and missense mutations and amplification of ARMCs account for most of them. In addition, ARMC5 and ARMC10 were independent prognostic factors in univariate and multivariate Cox regression analyses. Finally, through our confirmation experiment, it was found that the expression of ARMC1 and 10 in PAAD tissues was significantly increased compared with those in paracancer tissue.

CONCLUSION

This study suggests that ARMCs may be able to play important roles in PAAD, and they can act as biomarkers, providing valuable clues for the treatment and diagnosis of PAAD.

摘要

背景

胰腺腺癌（PAAD）的预后非常差，且针对PAAD的治疗方法很少。因此，寻找一些用于PAAD诊断和治疗的生物标志物很重要。尽管含犰狳重复序列蛋白（ARMCs）的某些成员已被证明与某些癌症的发生有关，但其与PAAD的关系仍不清楚。在本研究中，我们旨在探讨ARMCs在PAAD中的表达及预后价值。

方法

我们使用癌症基因组图谱（TCGA）数据库进行生存分析。然后，利用基因表达谱交互分析（GEPIA）、cBioPortal数据库、人类蛋白质图谱（HPA）、Kaplan-Meier Plotter、LinkedOmics数据库、基因本体论（GO）和京都基因与基因组百科全书（KEGG）、Cytoscape和Timer来分析ARMCs与PAAD之间的关系。此外，我们建立了PAAD的ARMCs预后模型。还进行了免疫组织化学（IHC）检测。然后使用Image-J分析从实验中获得的所有图像，并使用GraphPad-Prism（9.5.1）进行统计分析，以验证ARMCs在PAAD中的表达。

结果

在TCGA数据库中，PAAD组织中ARMC1、2、3、5、6、7、8、9和10的表达明显高于正常组织。ARMC1和10的高表达与PAAD患者较低的生存率相关。此外，ARMC2、5、6和10与PAAD的晚期呈正相关。11%的PAAD患者发生ARMCs突变，其中错义突变和ARMCs扩增占大多数。此外，在单因素和多因素Cox回归分析中，ARMC5和ARMC10是独立的预后因素。最后，通过我们的验证实验发现，与癌旁组织相比，PAAD组织中ARMC1和10的表达明显增加。