用于诊断儿童莱姆病的血浆微生物游离DNA测序

Plasma Microbial Cell-free DNA Sequencing for Diagnosis of Pediatric Lyme Disease.

作者信息

Handel Andrew S, Ahmed Asim A, Venkatasubrahmanyam Shivkumar, Bercovici Sivan, Mao Qing, Ho Carine, Hollemon Desiree D, Beneri Christy

机构信息

From the Division of Infectious Diseases, Department of Pediatrics, Stony Brook Children's Hospital, Stony Brook, New York.

Renaissance School of Medicine at Stony Brook University, Stony Brook, New York.

出版信息

Pediatr Infect Dis J. 2025 May 1;44(5):473-475. doi: 10.1097/INF.0000000000004707. Epub 2024 Dec 31.

DOI:10.1097/INF.0000000000004707

PMID:39774049

Abstract

We conducted an exploratory study of plasma microbial cell-free DNA sequencing for the diagnosis of Lyme disease among pediatric patients. Low levels of Borrelia burgdorferi microbial cell-free DNA (<3-5 molecules per microliter) were observed in 6/9 serologically confirmed participants, including 4/5 with arthritis and 2/3 with multiple erythema migrans.

摘要

我们开展了一项探索性研究，利用血浆微生物游离DNA测序诊断儿科患者的莱姆病。在9名血清学确诊的参与者中，6人检测到低水平的伯氏疏螺旋体微生物游离DNA（每微升<3 - 5个分子），其中包括5名患有关节炎的患者中的4人以及3名患有多处游走性红斑的患者中的2人。