Reisig Dominic, Huseth Anders
Department of Entomology and Plant Pathology, NC State University, Plymouth, NC, USA.
Department of Entomology and Plant Pathology and the North Carolina Plant Sciences Institute, NC State University, Raleigh, NC, USA.
J Econ Entomol. 2025 Feb 11;118(1):1-8. doi: 10.1093/jee/toae312.
Debate over resistance management tactics for genetically engineered (GE) crops expressing insecticidal toxins is not new. For several decades, researchers, regulators, and agricultural industry scientists have developed strategies to limit the evolution of resistance in populations of lepidopteran and coleopteran pests. A key attribute of many of these events was insecticide resistance management (IRM) strategies designed around a presumed high-dose expression sufficient to kill 99.5% of exposed larvae for some of the main target pests in corn, Zea mays L. and cotton, Gossypium hirsutum L. In contrast, other target pests did not meet this high-dose criterion. Similarly, the recent release of ThryvOn cotton that expresses thysanopteran and hemipteran active Mpp51Aa2.834_16 toxin is not high dose, working on a combination of behavioral and sublethal effects to suppress populations. This unique mode of control has generated considerable uncertainty about what IRM strategies will be most effective to limit field-evolved resistance to this unique spectrum of pests. The goal of this manuscript is to present several knowledge gaps that exist in proposed Mpp51Aa2 IRM plans, focusing on its activity on thrips, Frankliniella spp. Addressing these gaps will be crucial to limit resistance and preserve the benefits that this technology may provide by alleviating reliance on conventional insecticides and seed treatments. Broadly, these considerations will be important for future GE events that are non-high dose but remain valuable components of a more holistic insect management programs that integrate multiple tactics to reduce conventional insecticide use for challenging pests.
关于表达杀虫毒素的转基因作物抗性管理策略的争论并不新鲜。几十年来，研究人员、监管机构和农业行业科学家一直在制定策略，以限制鳞翅目和鞘翅目害虫种群中抗性的进化。许多这类事件的一个关键特性是围绕假定的高剂量表达设计的抗虫管理（IRM）策略，这种高剂量表达足以杀死玉米（Zea mays L.）和棉花（Gossypium hirsutum L.）中一些主要目标害虫99.5%的暴露幼虫。相比之下，其他目标害虫不符合这一高剂量标准。同样，最近推出的表达缨翅目和半翅目活性Mpp51Aa2.834_16毒素的ThryvOn棉花不是高剂量的，它通过行为和亚致死效应的组合来抑制种群。这种独特的控制方式使得关于哪种IRM策略最有效地限制田间对这种独特害虫谱的进化抗性产生了相当大的不确定性。本手稿的目的是指出拟议的Mpp51Aa2 IRM计划中存在的几个知识空白，重点关注其对蓟马（Frankliniella spp.）的活性。解决这些空白对于限制抗性和保持该技术通过减轻对传统杀虫剂和种子处理的依赖可能提供的益处至关重要。广泛地说，这些考虑对于未来非高剂量但仍是更全面的昆虫管理计划的重要组成部分的转基因事件很重要，这些计划整合多种策略以减少对具有挑战性害虫的传统杀虫剂使用。
