对摩洛哥人群中血管紧张素转换酶1（ACE1）D/I多态性及人口统计学因素与COVID-19结局相关性的首次评估。
The first assessment of Angiotensin-Converting Enzyme 1 (ACE1) D/I polymorphism and demographic factors in association with COVID-19 outcomes in the Moroccan Population.
作者信息
El Bouchikhi Ihssane, El Otmani Ihsane, Ahakoud Mohamed, Kettani Oussama, El Makhzen Badreddine, Yahyaoui Ghita, Oumokhtar Bouchra, Ouldim Karim, El-Azami-El-Idrissi Mohammed, Achour Sanae, Mahmoud Mustapha, Bouguenouch Laila
机构信息
Laboratory of Biomedical & Translational Research, Faculty of Medicine, Pharmacy and Dentistry of Fez, Sidi Mohamed Ben Abdellah University, BP 1893, Fez, 30070, Morocco.
Medical Genetics & Oncogenetics Laboratory, Hassan II University Hospital, Sidi Harazem Road, Fez, 30000, Morocco.
出版信息
Mol Biol Rep. 2025 Jan 8;52(1):109. doi: 10.1007/s11033-024-10211-6.
BACKGROUND
SARS-CoV-2 responsible for the COVID-19 pandemic, infiltrates the human body by binding to the ACE2 receptor in the respiratory system cell membranes, leading to severe lung tissue damage. An analog of ACE2, ACE1, has gained attention due to its well-known Deletion/Insertion (D/I) polymorphism, which seems to be associated with COVID-19 outcomes. This study aims to reveal the allelic and genotypic frequencies of the rs4646994 polymorphism in the Moroccan population and investigate the association between COVID-19 outcomes and both genotypic and demographic data.
METHODS AND RESULTS
We screened 162 Moroccan COVID-19 patients for the ACE1 gene D/I polymorphism using PCR amplification of the ACE1 polymorphic region within intron 16. Statistical analysis of the relationship between COVID-19 outcomes and each of the genetic and demographic data was performed using R software. The D allele was present in 74% of subjects. Homozygous (II) and heterozygous (DI) genotypes for the Insertion allele were present in 41.4% and 5.6% of patients, respectively. The median age in the COVID-19 'critical symptoms' category was significantly higher and gradually decreased with less severe symptoms. Similarly, males were significantly overrepresented in the 'critical symptoms' category, while females predominated in the 'mild symptoms' category.
CONCLUSIONS
The present study reports the prevalence of ACE1 D/I alleles for the first time in the Moroccan population and confirms the strong association of severe COVID-19 outcomes with male sex and older age. Moreover, this work is the first to explore the relationship between ACE1 D/I polymorphism and COVID-19 clinical outcomes in North African adults. The lack of a significant association may be due to cohort size or population-specific factors. A comprehensive investigation in a larger North African cohort is highly recommended.
背景
导致新冠疫情的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）通过与呼吸系统细胞膜中的血管紧张素转换酶2（ACE2）受体结合侵入人体，导致严重的肺组织损伤。ACE2的类似物ACE1因其众所周知的缺失/插入（D/I）多态性而受到关注，这种多态性似乎与新冠病毒病（COVID-19）的结局有关。本研究旨在揭示摩洛哥人群中rs4646994多态性的等位基因和基因型频率，并调查COVID-19结局与基因型和人口统计学数据之间的关联。
方法与结果
我们采用聚合酶链反应（PCR）扩增内含子16内的ACE1多态性区域，对162例摩洛哥COVID-19患者进行ACE1基因D/I多态性筛查。使用R软件对COVID-19结局与每种遗传和人口统计学数据之间的关系进行统计分析。74%的受试者存在D等位基因。插入等位基因的纯合子（II）和杂合子（DI）基因型分别出现在41.4%和5.6%的患者中。COVID-19“重症症状”组的中位年龄显著更高，且随着症状减轻而逐渐降低。同样，男性在“重症症状”组中的比例显著过高，而女性在“轻症症状”组中占主导。
结论
本研究首次报告了摩洛哥人群中ACE1 D/I等位基因的流行情况，并证实了重症COVID-19结局与男性性别和老年密切相关。此外，本研究首次探讨了北非成年人中ACE1 D/I多态性与COVID-19临床结局之间的关系。缺乏显著关联可能是由于队列规模或特定人群因素。强烈建议在更大的北非队列中进行全面调查。
Zotero 插件