Sun Caihua, Yao Hongliang, Liu Jipan, Wang Shuai
Department of General Surgery, Hengshui People's Hospital, China.
Department of Gynecology, Hengshui People's Hospital, China.
Adv Clin Exp Med. 2025 Aug;34(8):1375-1382. doi: 10.17219/acem/193399.
Some ADP ribosylation factors (ARF) and ADP ribosylation factor-like (ARL) family are involved in the regulation of certain cancers, but the role of ADP ribosylation factor-like 9 (ARL9) in gastric tumorigenesis remains elusive.
The main aim of this study was to evaluate the ARL9 expression within stomach cancer cells and elucidate its influence on the modulation of cancer cell behavior.
Differential ARL9 protein expression in normal stomach and stomach cancer tissue was ascertained through data sourced from the University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN). Quantitative analysis of ARL9 expression in gastric cancer tissue and its association with clinicopathological features was performed using quantitative polymerase chain reaction (qPCR) and western blot analysis (WB). Small interfering RNA (siRNA) was employed to suppress ARL9 protein expression in the human stomach gastric adenocarcinoma human gastric adenocarcinoma cells (AGS) cell line. Assessment of AGS gastric cancer (GC) cell proliferation, invasion and migration was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and transwell techniques.
The expression of ARL9 protein exhibited a significant upregulation in GC tissue, and showed a close association between tumor dimensions (p < 0.05) and the presence of distant metastases (p < 0.05) among individuals diagnosed with GC. However, no significant link was observed with sex, age and tumor-node-metastasis (TNM) staging in gastric malignancy patients. After the introduction of si-ARL9 in the experimental set, there was a noteworthy decrease in ARL9 protein levels in AGS cells (p < 0.01). In contrast to the control cohort, the restraint of ARL9 expression significantly hampered the growth, mobility and infiltration abilities of the AGS GC cell line (p < 0.01).
The significant correlation of ARL9 with the biological behavior of GC indicates its potentially pivotal role in the pathophysiology of the malignancy.
一些ADP核糖基化因子（ARF）和ADP核糖基化因子样（ARL）家族参与某些癌症的调控，但ADP核糖基化因子样9（ARL9）在胃癌发生中的作用仍不清楚。
本研究的主要目的是评估ARL9在胃癌细胞中的表达，并阐明其对癌细胞行为调节的影响。
通过阿拉巴马大学伯明翰分校癌症数据分析门户（UALCAN）的数据确定正常胃组织和胃癌组织中ARL9蛋白的差异表达。使用定量聚合酶链反应（qPCR）和蛋白质印迹分析（WB）对胃癌组织中ARL9的表达及其与临床病理特征的相关性进行定量分析。采用小干扰RNA（siRNA）抑制人胃腺癌AGS细胞系中ARL9蛋白的表达。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）和Transwell技术评估AGS胃癌（GC）细胞的增殖、侵袭和迁移能力。
ARL9蛋白在GC组织中的表达显著上调，在诊断为GC的个体中，肿瘤大小（p<0.05）与远处转移的存在（p<0.05）之间显示出密切关联。然而，在胃癌患者中，未观察到与性别、年龄和肿瘤-淋巴结-转移（TNM）分期有显著联系。在实验组中引入si-ARL9后，AGS细胞中ARL9蛋白水平显著降低（p<0.01）。与对照组相比，抑制ARL9表达显著阻碍了AGS GC细胞系的生长、迁移和浸润能力（p<0.01）。
ARL9与GC生物学行为的显著相关性表明其在恶性肿瘤病理生理学中可能起关键作用。
