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土耳其主要窄爪小龙虾（Eschscholtz，1823年）种群中小龙虾瘟疫病原体的流行情况、分子鉴定及基因分型

Prevalence, molecular identification and genotyping of the crayfish plague pathogen, in major narrow-clawed crayfish ( Eschscholtz, 1823) populations from Türkiye.

作者信息

Akhan Süleyman, Çağatay İfakat Tülay, Berber Selçuk, Taştan Büşra, Taştan Yiğit, Dalar Tuba

机构信息

Faculty of Fisheries, Akdeniz University, 07070 Antalya, Turkey.

Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, 17100 Çanakkale, Turkey.

出版信息

J Vet Res. 2024 Nov 6;68(4):515-524. doi: 10.2478/jvetres-2024-0061. eCollection 2024 Dec.

DOI:10.2478/jvetres-2024-0061
PMID:39776682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11702244/
Abstract

INTRODUCTION

Crayfish plague is considered the most important crayfish disease globally. It is caused by the fungus-like agent, . This study aimed to identify and determine the prevalence of using PCR in narrow-clawed crayfish () populations from across Türkiye.

MATERIAL AND METHODS

A PCR was carried out with primers specific to the internal transcribed spacer region of the pathogen on both telson and abdominal cuticle tissues from crayfish individuals from 41 different locations.

RESULTS

was detected in the crayfish from 34 of the locations. Molecular diagnosis showed the prevalence rates of to be between 0% and 68.2%. For 7 of the 34 locations, the strain of was determined. Microsatellite analysis of tissue from individuals with positive PCR results revealed the genotypes in seven populations. Genotype B was found to be the predominant genotype responsible for crayfish plague in Turkish crayfish populations. The Psl genotype (genotype B) was determined in six of the populations, and the As genotype (genotype A) was detected in only one.

CONCLUSION

Crayfish plague poses a significant threat to crayfish populations, necessitating the development of rapid, highly sensitive diagnostic methods. An understanding of the sensitivity of the PCR detection method and of the prevalence and genotyping of in Turkish crayfish populations has been gained from this study.

摘要

引言

小龙虾瘟疫被认为是全球最重要的小龙虾疾病。它由一种类似真菌的病原体引起。本研究旨在利用聚合酶链反应（PCR）鉴定并确定土耳其各地窄额螯虾（）种群中该病原体的流行情况。

材料与方法

使用针对该病原体内部转录间隔区的引物，对来自41个不同地点的小龙虾个体的尾节和腹部角质层组织进行PCR检测。

结果

在34个地点的小龙虾中检测到该病原体。分子诊断显示其流行率在0%至68.2%之间。对于34个地点中的7个，确定了该病原体的菌株。对PCR结果呈阳性的个体组织进行微卫星分析，在七个种群中揭示了该病原体的基因型。发现基因型B是导致土耳其小龙虾种群发生小龙虾瘟疫的主要基因型。在六个种群中确定为Psl基因型（基因型B），仅在一个种群中检测到As基因型（基因型A）。

结论

小龙虾瘟疫对小龙虾种群构成重大威胁，因此需要开发快速、高度灵敏的诊断方法。本研究已了解到PCR检测方法的灵敏度以及该病原体在土耳其小龙虾种群中的流行情况和基因分型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/6e731e6369cb/j_jvetres-2024-0061_fig_005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/eb062435ec31/j_jvetres-2024-0061_fig_001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/2a2092a063ff/j_jvetres-2024-0061_fig_002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/b14c2f4fca9c/j_jvetres-2024-0061_fig_003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/97e88f8611ec/j_jvetres-2024-0061_fig_004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/6e731e6369cb/j_jvetres-2024-0061_fig_005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/eb062435ec31/j_jvetres-2024-0061_fig_001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/2a2092a063ff/j_jvetres-2024-0061_fig_002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/b14c2f4fca9c/j_jvetres-2024-0061_fig_003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/97e88f8611ec/j_jvetres-2024-0061_fig_004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f605/11702244/6e731e6369cb/j_jvetres-2024-0061_fig_005.jpg

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