Liu Yuxi, Wang Sijin, Han Mengbo, Luo Sheng, Chu Peng, Tang Xiaodong, Zhao Cheng, Han Shiqun, Yin Shaowu, Wang Tao
College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China.
College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China.
Mar Pollut Bull. 2025 Mar;212:117533. doi: 10.1016/j.marpolbul.2025.117533. Epub 2025 Jan 7.
17α-methyltestosterone (17α-MT) is prevalent in the aqueous environment, but its toxicological profile remains incomplete. This study analyzed the effects of different 17α-MT concentrations on the growth performance, mortality, sex ratio, liver physiological metabolism, and intestinal microorganisms of Takifugu fasciatus, and on the microorganisms composition of its culture environment. Results showed that 17α-MT increased the male ratio and mortality but inhibited the growth of T. fasciatus. Meanwhile, 17α-MT induced oxidative stress and mitochondrial autophagy, changed lipid metabolism and inhibited triglyceride (TG) accumulation in the liver. 17α-MT altered the composition of the intestinal microorganisms, notably increasing the number of pathogenic bacteria. Additionally, the Firmicutes to the Bacteroidetes (F/B) ratio, associated with lipid metabolism, decreased with increasing 17α-MT concentration. Furthermore, 17α-MT disrupted the connection between the intestine microorganisms in T. fasciatus and the water column. This study enhances understanding of the effect of 17α-MT on T. fasciatus and the toxicological profile of 17α-MT.
17α-甲基睾酮（17α-MT）在水环境中普遍存在，但其毒理学特征仍不完整。本研究分析了不同浓度的17α-MT对暗纹东方鲀的生长性能、死亡率、性别比例、肝脏生理代谢、肠道微生物以及养殖环境中微生物组成的影响。结果表明，17α-MT提高了雄性比例和死亡率，但抑制了暗纹东方鲀的生长。同时，17α-MT诱导氧化应激和线粒体自噬，改变脂质代谢并抑制肝脏中甘油三酯（TG）的积累。17α-MT改变了肠道微生物的组成，显著增加了病原菌的数量。此外，与脂质代谢相关的厚壁菌门与拟杆菌门的比例（F/B）随着17α-MT浓度的增加而降低。此外，17α-MT破坏了暗纹东方鲀肠道微生物与水柱之间的联系。本研究增进了对17α-MT对暗纹东方鲀的影响以及17α-MT毒理学特征的理解。
