两种心肌肌钙蛋白T同工型结构的比较。
Comparison of the structure of two cardiac troponin T isoforms.
作者信息
Risnik V V, Verin A D, Gusev N B
出版信息
Biochem J. 1985 Jan 15;225(2):549-52. doi: 10.1042/bj2250549.
Abstract
Two isoforms of troponin T have been isolated from bovine cardiac muscle. One isoform has an Mr of 31000 and a pI at about 7.1, the corresponding values for the second isoform being 33000 and 6.5. Both isoforms have identical C- and N-terminal sequences, and, according to the data from tryptic-peptide mapping, a similar structure of the central and C-terminal domains. The large N-terminal peptides of troponin T isoforms differ in the content of glutamine/glutamic acid and alanine. It is concluded that the isoform with Mr 33000 has an additional peptide enriched with glutamic acid and alanine that is inserted between the N-terminal pentapeptide and the cysteine located 40-60 residues from the N-terminus.
摘要
已从牛心肌中分离出两种肌钙蛋白T同工型。一种同工型的相对分子质量为31000，等电点约为7.1，第二种同工型的相应值为33000和6.5。两种同工型的C端和N端序列相同，根据胰蛋白酶肽图谱数据，其中心结构域和C端结构域的结构相似。肌钙蛋白T同工型的大N端肽在谷氨酰胺/谷氨酸和丙氨酸含量上有所不同。得出的结论是，相对分子质量为33000的同工型有一个富含谷氨酸和丙氨酸的额外肽段，该肽段插入在N端五肽和距N端40 - 60个残基处的半胱氨酸之间。
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