Wang Ying, Wang Dagang, Huang Shouqiang, Wu Jie, Xiong Yuanzhen, Liu Fasheng, Shi Xiaowen, Liao Xinjun, Xiao Juhua, Zhang Shouhua, Lu Huiqiang
College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China.
Center for Clinical Medicine Research, Affiliated Hospital of Jinggangshan University, Jian 343000, Jiangxi Province, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2025 Mar;289:110123. doi: 10.1016/j.cbpc.2025.110123. Epub 2025 Jan 6.
The compound m-Cresol, also referred to as 3-methylphenol,acts as a precursor in the creation of pesticides and plasticizers. This research has conducted a thorough evaluation of the toxic effects of m-cresol on the cardiac development of juvenile zebrafish, from 6 to 72 hpf. The study's results reveal that higher concentrations of m-Cresol, compared to lower ones, result in more severe heart abnormalities in zebrafish larvae. The pericardial edema becomes more pronounced, the atrial-ventricular distance gradually increases, and the absorption of nutrients is delayed. Furthermore, experimental studies have shown that m-cresol can cause excessive oxidative stress and apoptosis in juvenile zebrafish during their early developmental stages. Additionally, our transcriptomic analysis indicates that m-Cresol exposure may cause cardiac developmental toxicity in zebrafish larvae by affecting the expression levels of genes (Myosin VIIa:my17,Myosin XIV:my14, Alpha-cardiac actin:actc1a,and Non-muscular myosin heavy chain 9 A:myh9a) involved in the ion channel signaling pathway and cardiomyocyte development. These findings collectively demonstrate the developmental toxicity of m-Cresol to the hearts of larval zebrafish.
化合物间甲酚，也被称为3-甲基苯酚，在农药和增塑剂的生产中用作前体。本研究对间甲酚在6至72小时胚胎期的斑马鱼幼体心脏发育过程中的毒性作用进行了全面评估。研究结果表明，与低浓度相比，高浓度的间甲酚会导致斑马鱼幼体出现更严重的心脏异常。心包水肿更加明显，心房-心室距离逐渐增加，营养物质的吸收延迟。此外，实验研究表明，间甲酚会在斑马鱼幼体早期发育阶段引起过度的氧化应激和细胞凋亡。此外，我们的转录组分析表明，接触间甲酚可能通过影响参与离子通道信号通路和心肌细胞发育的基因（肌球蛋白VIIa：my17、肌球蛋白XIV：my14、α-心肌肌动蛋白：actc1a和非肌肉肌球蛋白重链9A：myh9a）的表达水平，对斑马鱼幼体造成心脏发育毒性。这些发现共同证明了间甲酚对斑马鱼幼体心脏的发育毒性。
