Xiong Shifa, Wu Liwen, Chen Yicun, Shi Xiang, Wang Yangdong
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China; Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
J Proteomics. 2025 Mar 20;313:105373. doi: 10.1016/j.jprot.2024.105373. Epub 2025 Jan 6.
The ability of axillary meristems to form axillary buds and subsequently develop into branches is influenced by phytohormones, environmental conditions, and genetic factors. The main trunk of Quercus fabri is prone to branching, which not only impacts the appearance and density of the wood and significantly reduces the yield rate. This study conducted transcriptomic, proteomic, and metabolomic analyses on three stages of axillary bud development in Q. fabri. A total of 12,888 differentially expressed genes (DEGs), 8193 differentially accumulated proteins (DAPs), and 1788 differentially accumulated metabolites (DAMs) were identified through comparisons among the stages and subjected to multi-omics joint analysis. Conduct interaction network analysis on DEGs and DAPs to identify the significant transcription factor family (AP2/ERF) involved in the regulation of axillary bud development. Furthermore, KEGG enrichment analysis of DEGs, DAPs and DAMs indicated significant enrichment in plant hormone signaling pathways. The analysis of endogenous hormone levels and qRT-PCR results for pathway genes demonstrated that the expression levels of IAA and tZ significantly increased during late developmental stages, whereas the expression levels of ABA, ACC and JA significantly decreased. In summary, these findings contribute to a comprehensive understanding of the regulatory networks underlying the branching development of Q. fabri. SIGNIFICANCE: Q. fabri exhibits robust vegetative growth, and its primary trunk is prone to branching, significantly influencing the wood yield rate. Through a joint analysis of transcriptomics, proteomics, and metabolomics, we comprehensively examined the regulatory network governing the axillary bud development of Q. fabri. Our findings revealed the crucial roles of the AP2/ERF transcription factor family and plant hormone signal transduction pathways in branch development. These insights contribute to a deeper understanding of the mechanisms regulating branch development.
腋生分生组织形成腋芽并随后发育成枝条的能力受植物激素、环境条件和遗传因素的影响。麻栎的主干易于分枝，这不仅影响木材的外观和密度，还会显著降低出材率。本研究对麻栎腋芽发育的三个阶段进行了转录组学、蛋白质组学和代谢组学分析。通过各阶段之间的比较，共鉴定出12888个差异表达基因（DEG）、8193个差异积累蛋白（DAP）和1788个差异积累代谢物（DAM），并进行了多组学联合分析。对DEG和DAP进行相互作用网络分析，以鉴定参与腋芽发育调控的重要转录因子家族（AP2/ERF）。此外，对DEG、DAP和DAM的KEGG富集分析表明，植物激素信号通路显著富集。对内源激素水平和通路基因的qRT-PCR结果分析表明，IAA和tZ的表达水平在发育后期显著增加，而ABA、ACC和JA的表达水平显著降低。总之，这些发现有助于全面了解麻栎分枝发育的调控网络。意义：麻栎表现出旺盛的营养生长，其主干易于分枝，显著影响木材出材率。通过转录组学、蛋白质组学和代谢组学的联合分析，我们全面研究了麻栎腋芽发育的调控网络。我们的发现揭示了AP2/ERF转录因子家族和植物激素信号转导通路在枝条发育中的关键作用。这些见解有助于更深入地理解调控枝条发育的机制。
