Le Xiao-Na, Long Da-Ping, Yin Shuang-Shuang, Qing Ren-Yan, Chi Zhi-Zheng, Gao Ming-Qing, Zhu Ming-Qiang
College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling 712100, China; Western Scientific Observation and Experiment Station for Development and Utilization of Rural Renewable Energy, M.O.A, Northwest Agriculture & Forestry University, Yangling 712100, China.
College of Forestry, Northwest A&F University, Yangling 712100, China; Western Scientific Observation and Experiment Station for Development and Utilization of Rural Renewable Energy, M.O.A, Northwest Agriculture & Forestry University, Yangling 712100, China.
Carbohydr Polym. 2025 Mar 1;351:123100. doi: 10.1016/j.carbpol.2024.123100. Epub 2024 Dec 2.
The efficient extraction and purification of active components from Eucommia ulmoides Oliver (EUO) are crucial for their utilization. The structure and properties of the prepared EUO leaf polysaccharides (ELPs) and extractum (ELE) were comprehensively characterized in this study, and the intervention mechanism of the EUO polysaccharides and extractum in alcoholic liver disease (ALD) were investigated. The yield of EUO extractum was 24.82 %, from which nine active components were identified. The yield of EUO leaf polysaccharides was 8.06 %, and the polysaccharides were fractionated into three components ELP1, ELP2, and ELP3 through ultrafiltration technology, with yields of 4.19 %, 1.26 %, and 2.59 %, respectively. Ultrafiltration significantly reduced protein content, enhanced polysaccharide homogeneity, and altered monosaccharide composition. ELP3 exhibited higher scavenging efficacy on •OH and ABTS• than ELP1 and ELP2, reaching 82.53 % and 88.41 % respectively. ELP3 and ELE intervention preserved liver integrity, mitigated lipid accumulation and inflammation, and regulated hepatic oxidative stress. Moreover, they maintained intestinal barrier function, suppressed harmful bacteria (Escherichia-Shigella, and UBA1819), and promoted beneficial bacteria (Dubosiella, Monoglobus, and Lachnospiraceae). Thirteen hallmark differential metabolites were identified, and KEGG pathway enrichment analysis suggested that ELP3 and ELE may ameliorate ALD through pathways like longevity regulation, choline metabolism in cancer, oxidative phosphorylation, and AMPK signaling pathway. This investigation holds significance in delineating the beneficial effects of ELP3 and ELE in ALD alleviation.
从杜仲（Eucommia ulmoides Oliver，EUO）中高效提取和纯化活性成分对其利用至关重要。本研究对制备的杜仲叶多糖（ELPs）和提取物（ELE）的结构与性质进行了全面表征，并研究了杜仲多糖和提取物在酒精性肝病（ALD）中的干预机制。杜仲提取物的得率为24.82%，从中鉴定出9种活性成分。杜仲叶多糖的得率为8.06%，通过超滤技术将多糖分离为三个组分ELP1、ELP2和ELP3，得率分别为4.19%、1.26%和2.59%。超滤显著降低了蛋白质含量，提高了多糖的均一性，并改变了单糖组成。ELP3对•OH和ABTS•的清除效果高于ELP1和ELP2，分别达到82.53%和88.41%。ELP3和ELE干预可维持肝脏完整性，减轻脂质蓄积和炎症，并调节肝脏氧化应激。此外，它们还能维持肠道屏障功能，抑制有害菌（大肠埃希氏菌-志贺氏菌属和UBA1819），并促进有益菌（Dubosiella、Monoglobus和毛螺菌科）。鉴定出13种标志性差异代谢物，KEGG通路富集分析表明，ELP3和ELE可能通过寿命调控、癌症中的胆碱代谢、氧化磷酸化和AMPK信号通路等途径改善ALD。本研究对于阐明ELP3和ELE在减轻ALD方面的有益作用具有重要意义。
