Schölmerich J, Becher M S, Baumgartner U, Gerok W
Biochem Biophys Res Commun. 1985 Feb 15;126(3):1146-53. doi: 10.1016/0006-291x(85)90305-5.
Bile acids induce membrane alterations including reduced response to peptide hormones in vitro. Isolated liver cells from rats with bile duct obstruction were studied regarding gluconeogenesis and its hormonal control. While cells from shamoperated animals showed an 63% increase of glucose release in the presence of glucagon (1 microM), cells from cholestatic livers did not response regardless of the duration of obstruction. Cholestatic cells also showed other signs of membrane alterations, such as an increased enzyme leakage while redoxstatus and other metabolic responses were unchanged. These results suggest that a loss of hormonal control in the liver could contribute to disturbations of glucose homeostasis in cholestatic conditions.
胆汁酸在体外可诱导包括对肽类激素反应降低在内的膜改变。对胆管阻塞大鼠的分离肝细胞进行了有关糖异生及其激素调控的研究。假手术动物的细胞在胰高血糖素（1微摩尔）存在时葡萄糖释放增加63%，而无论阻塞时间长短，胆汁淤积肝脏的细胞均无反应。胆汁淤积细胞还表现出其他膜改变的迹象，如酶泄漏增加，而氧化还原状态和其他代谢反应未改变。这些结果表明，肝脏中激素调控的丧失可能导致胆汁淤积状态下葡萄糖稳态的紊乱。
