Cunha Diogo M, Hernández-Pérez Sara, Mattila Pieta K
Institute of Biomedicine and MediCity Research Laboratories, University of Turku, Turku, Finland.
InFLAMES Research Flagship, University of Turku, Turku, Finland.
Sci Rep. 2025 Jan 8;15(1):1348. doi: 10.1038/s41598-024-83954-8.
CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.
CD19-Cre是一种重要且广泛应用于小鼠系统中B细胞特异性基因操作的Cre-lox模型。携带一个CD19-Cre等位基因的小鼠同时成为B细胞抗原受体（BCR）的关键共受体CD19的杂合子。因此，CD19-Cre小鼠表现出CD19表达水平降低，这对B细胞活化有潜在影响，但尚未得到充分研究。在此，我们报告，在依赖T细胞（NP-KLH）和不依赖T细胞（NP-Ficoll）免疫后，CD19-Cre小鼠的抗体反应显著增强，同时基础IgM免疫球蛋白水平也升高。在体外，我们观察到在LPS和IFNγ刺激下，类别转换重组增强，B细胞增殖适度减少，但BCR信号传导没有显著差异。我们的研究结果值得在B细胞研究中使用CD19-Cre小鼠模型时予以仔细考虑。
