Reveguk Tamara, Fatiukha Andrii, Potapenko Evgenii, Reveguk Ivan, Sela Hanan, Klymiuk Valentyna, Li Yinghui, Pozniak Curtis, Wicker Thomas, Coaker Gitta, Fahima Tzion
Institute of Evolution, University of Haifa, Haifa, Israel.
Department of Evolutionary and Environmental Biology, University of Haifa, Haifa, Israel.
Nat Genet. 2025 Jan;57(1):254-262. doi: 10.1038/s41588-024-02032-x. Epub 2025 Jan 8.
Plant pathogens pose a continuous threat to global food production. Recent discoveries in plant immunity research unveiled a unique protein family characterized by an unusual resistance protein structure that combines two kinase domains. This study demonstrates the widespread occurrence of tandem kinase proteins (TKPs) across the plant kingdom. An examination of 104 plant species' genomes uncovered 2,682 TKPs. The majority (95.6%) of these kinase domains are part of the receptor-like kinase-Pelle family, which is crucial for cell surface responses in plant immunity. Notably, 90% of TKPs comprise dual kinase domains, with over 50% being pseudokinases. Over 56% of these proteins harbor 127 different integrated domains, and over 47% include a transmembrane domain. TKP pseudokinases and/or integrated domains probably serve as decoys, engaging with pathogen effectors to trigger plant immunity. The TKP Atlas we created sheds light on the mechanisms of TKP convergent molecular evolution and potential function.
植物病原体对全球粮食生产构成持续威胁。植物免疫研究中的最新发现揭示了一个独特的蛋白质家族，其特征是具有一种不同寻常的抗性蛋白结构，该结构结合了两个激酶结构域。这项研究表明串联激酶蛋白（TKP）在植物界广泛存在。对104种植物物种的基因组进行检查后发现了2682个TKP。这些激酶结构域中的大多数（95.6%）是类受体激酶-佩尔家族的一部分，该家族对植物免疫中的细胞表面反应至关重要。值得注意的是，90%的TKP包含双激酶结构域，其中超过50%是假激酶。这些蛋白质中超过56%含有127种不同的整合结构域，超过47%包含一个跨膜结构域。TKP假激酶和/或整合结构域可能充当诱饵，与病原体效应子相互作用以触发植物免疫。我们创建的TKP图谱揭示了TKP趋同分子进化的机制和潜在功能。
