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通过与磺酞染料衍生化测定六种二氢吡啶类钙通道阻滞剂的新型环保分光光度法：应用于片剂分析

Novel ecofriendly spectrophotometric methods for the determination of six dihydropyridines calcium channel blockers through derivatization with sulfophtalein dye: application to tablet analysis.

作者信息

Horyn Mariana, Kryskiw Liubomyr, Kucher Tetyana, Zarivna Nadiya, Poliak Olha, Logoyda Liliya

机构信息

Department of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine.

出版信息

BMC Chem. 2025 Jan 8;19(1):8. doi: 10.1186/s13065-024-01378-x.

DOI:10.1186/s13065-024-01378-x
PMID:39780296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11716373/
Abstract

Novel, «green» and simple visible spectrophotometric procedures for the determination of six dihydropyridines CCBs (amlodipine besylate (AML), lacidipine (LAC), levamlodipine besylate (LAML), nifedipine (NIF), nimodipine (NIM) and nitrendipine (NIT)) through derivatization with the sulfophthalein dye bromophenol blue (BPB) have been developed. The optimal parameters for CCBs spectrophotometric analysis via complex formation using BPB were as follows: detection wavelength-596 nm, reaction time-5 min, ratio of reacting components-1:1, operating temperature-25 ± 2 °C. The concentration was linearly proportional to absorbance values in the range of 3.40-17.00 μg/mL (AML), 1.14-9.11 μg/mL (LAC), 1.14-9.08 μg/mL (LAML), 4.16-12.40 μg/mL (NIF), 0.84-5.86 μg/mL (NIM), 6.52-19.60 μg/mL (NIT). The developed methods are colorimetric and therefore does not require a UV instrument to quantify these drugs. The proposed approach was more efficient in terms of time reliability, sensitivity and «greenness» than other recorded spectrophotometric methods and can be easily implemented for routine pharmaceutical analysis.

摘要

已开发出新颖、“绿色”且简单的可见分光光度法，通过与磺酞染料溴酚蓝（BPB）衍生化来测定六种二氢吡啶类钙通道阻滞剂（苯磺酸氨氯地平（AML）、拉西地平（LAC）、苯磺酸左旋氨氯地平（LAML）、硝苯地平（NIF）、尼莫地平（NIM）和尼群地平（NIT））。使用BPB通过络合物形成进行钙通道阻滞剂分光光度分析的最佳参数如下：检测波长 - 596 nm，反应时间 - 5分钟，反应组分比例 - 1:1，操作温度 - 25 ± 2°C。浓度在3.40 - 17.00 μg/mL（AML）、1.14 - 9.11 μg/mL（LAC）、1.14 - 9.08 μg/mL（LAML）、4.16 - 12.40 μg/mL（NIF）、0.84 - 5.86 μg/mL（NIM）、6.52 - 19.60 μg/mL（NIT）范围内与吸光度值呈线性比例关系。所开发的方法是比色法，因此不需要紫外仪器来定量这些药物。与其他已记录的分光光度法相比，所提出的方法在时间可靠性、灵敏度和“绿色度”方面更有效，并且可以很容易地应用于常规药物分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/f94d4ab52dd6/13065_2024_1378_Fig13_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/820a0d9933fd/13065_2024_1378_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/6e745bcc7a23/13065_2024_1378_Fig9_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/15d031f9cad4/13065_2024_1378_Fig11_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/f94d4ab52dd6/13065_2024_1378_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/689664cde4a3/13065_2024_1378_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/1336a80393ab/13065_2024_1378_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/c140c2acf6a2/13065_2024_1378_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/1a972932c2f8/13065_2024_1378_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/d68bad4c712c/13065_2024_1378_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/c99805b003de/13065_2024_1378_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/38ce1630fda1/13065_2024_1378_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/820a0d9933fd/13065_2024_1378_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/6e745bcc7a23/13065_2024_1378_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/6a0a14d35cb5/13065_2024_1378_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/15d031f9cad4/13065_2024_1378_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/40f276e7134a/13065_2024_1378_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba0c/11716373/f94d4ab52dd6/13065_2024_1378_Fig13_HTML.jpg

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