Zhou Daqian, Zhang Xingrui, Lv Jiale, Mei Yongliang, Luo Yingjin, Li Fengjiang, Liu Zongchao
Department of Orthopedics, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou Sichuan China.
Department of Orthopedics The First People's Hospital of Liangshan YiAutonomous Prefecture Liangshan Sichuan China.
JOR Spine. 2025 Jan 8;8(1):e70032. doi: 10.1002/jsp2.70032. eCollection 2025 Mar.
Intervertebral disc degeneration disease (IVDD) is a prevalent orthopedic condition that causes chronic lower back pain, imposing a substantial economic burden on patients and society. Despite its high incidence, the pathophysiological mechanisms of IVDD remain incompletely understood.
This study aimed to identify metabolomic alterations in IVDD patients and explore the key metabolic pathways and metabolites involved in its pathogenesis.
Serum samples from 20 IVDD patients and 20 healthy controls were analyzed using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS). The identified metabolites were mapped to metabolic pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database.
Significant alterations were observed in metabolites such as 2-methyl-1,3-cyclohexadiene, stearoyl sphingomyelin, methylcysteine, L-methionine, and cis, cis-muconic acid. These metabolites were involved in pathways including glycine, serine, and threonine metabolism, cyanoamino acid metabolism, and the citrate cycle (TCA cycle).
The identified metabolic alterations provide insights into the pathogenesis of IVDD and suggest potential therapeutic targets for future investigation.
椎间盘退变疾病（IVDD）是一种常见的骨科疾病，可导致慢性下腰痛，给患者和社会带来沉重的经济负担。尽管其发病率很高，但IVDD的病理生理机制仍未完全了解。
本研究旨在确定IVDD患者的代谢组学改变，并探索其发病机制中涉及的关键代谢途径和代谢物。
使用超高效液相色谱-质谱联用仪（UHPLC-MS）分析20例IVDD患者和20例健康对照者的血清样本。使用京都基因与基因组百科全书（KEGG）数据库将鉴定出的代谢物映射到代谢途径。
观察到2-甲基-1,3-环己二烯、硬脂酰鞘磷脂、甲基半胱氨酸、L-蛋氨酸和顺式，顺式-粘康酸等代谢物有显著改变。这些代谢物参与了甘氨酸、丝氨酸和苏氨酸代谢、氰基氨基酸代谢以及柠檬酸循环（TCA循环）等途径。
所确定的代谢改变为IVDD的发病机制提供了见解，并为未来的研究提出了潜在的治疗靶点。
