Zhang Tongxia, Zhang Tao, Yu Han, Chi Lingyi
Research Institute of Neuromuscular and Neurodegenerative Diseases and Department of Neurology, Qilu Hospital of Shandong University, Jinan, Shandong, People's Republic of China.
School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
Immun Inflamm Dis. 2025 Jan;13(1):e70110. doi: 10.1002/iid3.70110.
Traumatic spinal cord injury (SCI) is an incurable condition that is the largest cause of disability. In previous studies, Isosteviol sodium (STVNa) has been shown to protect rats against acute focal cerebral ischemia; however, the effects of STVNa on SCI recovery in rats remain unknown.
STVNa was given intraperitoneally after SCI to see if it had any neuroprotective benefits. On Days 7, 14, 21, and 28 post-SCI, functional recovery was measured using the Basso, Beattie, and Bresnahan (BBB) scoring system along with the oblique plate test. Following these evaluations, spinal cord tissues were harvested for analysis. All behavioral testing occurred between 8 a.m. and 3 p.m.
We found that STVNa improved spinal cord functional recovery in rats, as evidenced by enhanced BBB locomotor rating scale, angle of inclination, decreased cavity of spinal cord damage, and neuron death in vivo. In addition, STVNa reduced inflammation in rats following SCI, as demonstrated by a reduction in proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and interleukin (IL)-1β. STVNa also reduced oxidative damage in SCI rats by lowering ROS while raising SOD levels.
These findings suggest that STVNa protects SCI rats through a variety of pathways. STVNa, in particular, may benefit the recovery of SCI by reducing oxidative stress and inflammation, leading to enhanced locomotor activity in rats with SCI.
创伤性脊髓损伤（SCI）是一种无法治愈的疾病，是导致残疾的最大原因。在先前的研究中，异甜菊醇钠（STVNa）已被证明可保护大鼠免受急性局灶性脑缺血的影响；然而，STVNa对大鼠脊髓损伤恢复的影响尚不清楚。
在脊髓损伤后腹腔注射STVNa，观察其是否具有神经保护作用。在脊髓损伤后的第7、14、21和28天，使用Basso、Beattie和Bresnahan（BBB）评分系统以及斜板试验来测量功能恢复情况。在这些评估之后，采集脊髓组织进行分析。所有行为测试均在上午8点至下午3点之间进行。
我们发现STVNa改善了大鼠脊髓功能的恢复，体内增强的BBB运动评分量表、倾斜角度、脊髓损伤空洞的减小以及神经元死亡证明了这一点。此外，STVNa减少了脊髓损伤后大鼠的炎症，如肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和白细胞介素（IL）-1β等促炎细胞因子的减少证明了这一点。STVNa还通过降低活性氧（ROS）同时提高超氧化物歧化酶（SOD）水平，减少了脊髓损伤大鼠的氧化损伤。
这些发现表明，STVNa通过多种途径保护脊髓损伤大鼠。特别是，STVNa可能通过减少氧化应激和炎症来促进脊髓损伤的恢复，从而增强脊髓损伤大鼠的运动活性。
