应用固定化多靶点磁珠对复杂天然产物中的生物活性成分进行新型选择性配体提取。
Noval Selective Ligand-Extraction of Bioactive Components in Complex Natural Products Applying Immobilized Multi-Target Magnetic Beads.
作者信息
Wang Chen, Qin Xueying, Wang Ying, Wang Weiping, Sun Shuai, Wang Hongsen, Li Shiyu, Dai Xiao, Zhu Xianhong, Gao Xun, Qin Kunming, Zhang Qing
机构信息
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China.
Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Ocean University, Lianyungang, China.
出版信息
Biomed Chromatogr. 2025 Feb;39(2):e6060. doi: 10.1002/bmc.6060.
Choerospondias axillaris is a medicinal plant used for treating coronary heart disease (CHD) due to its broad spectrum of anti-inflammatory activities. Cyclooxygenase 2 (COX-2) and lipoxygenase 5 (5-LOX) were immobilized on magnetic nanoparticles for selective ligand-extraction of these two enzymes present in C. axillaris. Sixteen extracted components were identified and analyzed using ultraperformance liquid chromatography plus Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-ExactiveOrbitrap-MS/MS), including flavonoids Curcumin and Epicatechin. The metabolization of the aforementioned 16 components in rats with acute blood stasis indicated a solid pharmacodynamic foundation. This method offers an effective approach for conducting basic research on the enrichment, analysis, and efficacy evaluation of active components in complex natural products.
南酸枣是一种药用植物，因其具有广泛的抗炎活性而用于治疗冠心病（CHD）。将环氧化酶2（COX-2）和脂氧合酶5（5-LOX）固定在磁性纳米颗粒上，用于选择性提取南酸枣中存在的这两种酶。使用超高效液相色谱联用Q-Exactive Orbitrap串联质谱（UHPLC-Q-Exactive Orbitrap-MS/MS）对16种提取成分进行了鉴定和分析，包括黄酮类化合物姜黄素和表儿茶素。上述16种成分在急性血瘀大鼠中的代谢情况表明了其坚实的药效学基础。该方法为开展复杂天然产物中活性成分的富集、分析及功效评价等基础研究提供了一种有效途径。
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