大鼠肝癌及肝癌发生过程中的癌基因表达。

Oncogene expression in rat hepatomas and during hepatocarcinogenesis.

作者信息

Cote G J, Lastra B A, Cook J R, Huang D P, Chiu J F

出版信息

Cancer Lett. 1985 Mar;26(2):121-7. doi: 10.1016/0304-3835(85)90017-5.

Abstract

In this study we examined oncogene expression in several Morris hepatomas. We found elevated transcripts for Ha-ras, Ki-ras, and the myc oncogenes, while the src oncogene was not increased. Northern analysis revealed transcript sizes of 1.4 kb for Ha-ras and 2.5 kb for myc. Analysis of primary tumors induced by the carcinogen 3'-methyl-4-dimethylaminoazobenzene (3'-MDAB) showed increasing oncogene transcript production with increasing length of diet. The results show enhanced ras and myc expression in rat hepatocellular carcinomas and during hepatocarcinogenesis.

摘要

在本研究中，我们检测了几种莫里斯肝癌中的癌基因表达情况。我们发现Ha-ras、Ki-ras和myc癌基因的转录本水平升高，而src癌基因的转录本水平未升高。Northern印迹分析显示，Ha-ras的转录本大小为1.4 kb，myc的转录本大小为2.5 kb。对由致癌物3'-甲基-4-二甲基氨基偶氮苯（3'-MDAB）诱导的原发性肿瘤进行分析，结果显示，随着喂食时间的延长，癌基因转录本的产生量增加。这些结果表明，在大鼠肝细胞癌及肝癌发生过程中，ras和myc的表达增强。