Yang L Y, Drewinko B
Cancer Res. 1985 Apr;45(4):1511-5.
The stability of the cell-killing efficacy of 10 antitumor drugs stored at different temperatures and concentrations was evaluated on an established human colon carcinoma cell line using the colony formation technique. Drugs were reconstituted in the appropriate solvent, further diluted in 0.9% NaCl solution to obtain the desired storage concentration, and stored at 4 degrees C, -20 degrees C, and -70 degrees C for periods of up to 3 weeks. Cytotoxic efficacy was tested weekly by exposing the cells to the final drug dilution for 1 h at 37 degrees C. Stability of cytotoxic potency depended on the particular drug, and the duration, concentration, and temperature of storage but, in general, most drugs remained efficacious only within limited periods of time (at most, 2 weeks). These results suggest that the conditions of drug storage following reconstitution are critical factors with respect to lethal efficacy. Therefore, analysis of in vitro cytotoxic efficacy can be meaningful only when storage conditions are specified in order to avoid false-negative results in the human tumor clonogenic assay.
使用集落形成技术，在已建立的人结肠癌细胞系上评估了10种抗肿瘤药物在不同温度和浓度下储存时细胞杀伤效力的稳定性。药物用适当的溶剂复溶，再在0.9%氯化钠溶液中进一步稀释以获得所需的储存浓度，并在4℃、-20℃和-70℃下储存长达3周。每周通过将细胞在37℃下暴露于最终药物稀释液1小时来测试细胞毒性效力。细胞毒性效力的稳定性取决于特定药物以及储存的持续时间、浓度和温度，但一般来说，大多数药物仅在有限的时间段内（最多2周）保持有效。这些结果表明，复溶后药物的储存条件是影响致死效力的关键因素。因此，只有在指定储存条件以避免人肿瘤克隆形成试验中出现假阴性结果时，体外细胞毒性效力分析才有意义。
