Wang Hongming
Department of Ear, Nose and Throat (ENT), The First People's Hospital of Jiande, No. 599 Yanzhou Avenue, Xin'anjiang Street, Jiande, 311600, Zhejiang, China.
Discov Oncol. 2025 Jan 9;16(1):27. doi: 10.1007/s12672-025-01772-4.
To screen potential differentially expressed genes related to immune function in nasopharyngeal carcinoma through an online database, and to verify their mechanism of action, so as to provide a reference for the diagnosis and treatment of nasopharyngeal carcinoma in the future.
Differentially expressed genes were analyzed from the GSE227541 dataset, and functional enrichment analysis was conducted. With mucin 5B, oligomeric mucus/gel-forming as the focus, the correlation between its expression and immune indexes was analyzed by using the TIMER database. The expressions of mucin 5B, oligomeric mucus/gel-forming in clinical NPC tissues and adjacent tissue samples were detected Furthermore, mucin 5B, oligomeric mucus/gel-forming abnormal expression vectors were constructed and transfected into human NPC cell CNE-2Z to detect alterations in cell activity, ferroptosis and the immune microenvironment. In addition, the role of the Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma was observed, and the influence of mucin 5B, oligomeric mucus/gel-forming on this pathway was discussed.
A total of 42 differentially expressed genes were found in the GSE227541 dataset, among which mucin 5B, oligomeric mucus/gel-forming was obviously at the core of the entire network. In nasopharyngeal carcinoma tissues, the research team observed the upregulated expression of mucin 5B, oligomeric mucus/gel-forming (P < 0.05). In vitro, increased expression of elevated mucin 5B, oligomeric mucus/gel-forming activates nasopharyngeal carcinoma cell activity and immune escape and inhibits ferroptosis. In terms of pathways, upregulating mucin 5B, oligomeric mucus/gel-forming expression could activate the Wnt/β-catenin pathway.
Mucin 5B, oligomeric mucus/gel-forming regulates the immune escape of nasopharyngeal carcinoma cells and participates in tumor progression by mediating the Wnt/β-catenin signaling pathway.
通过在线数据库筛选鼻咽癌中与免疫功能相关的潜在差异表达基因，并验证其作用机制，为未来鼻咽癌的诊断和治疗提供参考。
从GSE227541数据集中分析差异表达基因，并进行功能富集分析。以粘蛋白5B、寡聚黏液/凝胶形成蛋白为重点，利用TIMER数据库分析其表达与免疫指标之间的相关性。此外，检测临床鼻咽癌组织和癌旁组织样本中粘蛋白5B、寡聚黏液/凝胶形成蛋白的表达情况。构建粘蛋白5B、寡聚黏液/凝胶形成蛋白异常表达载体并转染至人鼻咽癌CNE-2Z细胞，检测细胞活性、铁死亡及免疫微环境的变化。另外，观察Wnt/β-连环蛋白信号通路在鼻咽癌中的作用，并探讨粘蛋白5B、寡聚黏液/凝胶形成蛋白对该通路的影响。
在GSE227541数据集中共发现42个差异表达基因，其中粘蛋白5B、寡聚黏液/凝胶形成蛋白明显处于整个网络的核心位置。在鼻咽癌组织中，研究团队观察到粘蛋白5B、寡聚黏液/凝胶形成蛋白表达上调（P＜0.05）。在体外，粘蛋白5B、寡聚黏液/凝胶形成蛋白表达升高可激活鼻咽癌细胞活性和免疫逃逸，并抑制铁死亡。在信号通路上，上调粘蛋白5B、寡聚黏液/凝胶形成蛋白的表达可激活Wnt/β-连环蛋白通路。
粘蛋白5B、寡聚黏液/凝胶形成蛋白通过介导Wnt/β-连环蛋白信号通路调节鼻咽癌细胞的免疫逃逸并参与肿瘤进展。
