用于高级细胞外囊泡检测的基于表面增强拉曼散射（SERS）的生物传感器：综述

Surface-Enhanced Raman Scattering (SERS)-based biosensors for advanced extracellular vesicle detection: A review.

作者信息

Fan Rui, Chen Siting, Lan Fei, Li Wenbin, Zhu Yitong, Zhang Lifeng, Zhang Ye, Li Ling

机构信息

School of Medical Technology, Guangdong Medical University, Dongguan, 523808, China; Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China.

Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China; School of Basic Medical Science, Southern Medical University, Guangzhou, 510515, China.

出版信息

Anal Chim Acta. 2025 Jan 22;1336:343264. doi: 10.1016/j.aca.2024.343264. Epub 2024 Sep 27.

DOI:10.1016/j.aca.2024.343264

PMID:39788643

Abstract

BACKGROUND

Extracellular Vesicles (EVs), as nano-scale vesicles rich in biological information, hold an indispensable status in the biomedical field. However, due to the intrinsic small size and low abundance of EVs, their effective detection presents significant challenges. Although various EV detection techniques exist, their sensitivity and ease of operation still need enhancement.

RESULTS

Surface-Enhanced Raman Scattering (SERS) is known for its high sensitivity and specificity. It stands out in tackling the challenges that traditional EV detection methods face. In this review, we focus on the application of SERS-based biosensors in EV detection. It provides a detailed introduction to the recognition and capture of EVs, strategies for mediating signal amplification, and detection of EV biomarkers. Finally, the challenges and prospects of SERS-based biosensors are discussed.

SIGNIFICANCE

SERS-based biosensor enhances the Raman signal, allowing for the detection of biomarkers at low concentrations. This capability reveals its substantial potential in identifying EVs and analyzing molecular data. It paves the path for advanced EV detection.

摘要

背景

细胞外囊泡（EVs）作为富含生物信息的纳米级囊泡，在生物医学领域具有不可或缺的地位。然而，由于EVs本身尺寸小且丰度低，其有效检测面临重大挑战。尽管存在各种EV检测技术，但其灵敏度和操作简便性仍需提高。

结果

表面增强拉曼散射（SERS）以其高灵敏度和特异性而闻名。它在应对传统EV检测方法面临的挑战方面脱颖而出。在本综述中，我们重点关注基于SERS的生物传感器在EV检测中的应用。它详细介绍了EVs的识别和捕获、介导信号放大的策略以及EV生物标志物的检测。最后，讨论了基于SERS的生物传感器面临的挑战和前景。