Yilmaz Osman, Arora Kshtij, Lee Soo Hyun, Hosseini Sahar, Chen Feidi, Padmanabha Nandan, Eng George, Kantekure Kanchan, Yilmaz Omer, Deshpande Vikram
Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
Massachusetts General Hospital, Wuincy, Massachusetts, USA.
J Clin Pathol. 2025 Jan 9. doi: 10.1136/jcp-2024-209856.
WNT signalling pathway dysregulation is often a critical early component in colorectal neoplasia, particularly the chromosomal instability pathway. Using two WNT reporters, and , we sought to assess whether these polyps demonstrate predictable expression patterns and if these patterns show diagnostic value.
We evaluated 23 adenomas (TA), 23 sessile serrated lesions (SSLs), 14 SSL with dysplasia and 38 traditional serrated adenomas (TSA). Chromogenic in situ hybridisation stains (ISH) for and were performed. Reactivity was defined as strong, intermediate or weak. Upper third crypt reactivity was defined as full-thickness staining. Accentuation within ectopic crypts (ECF) was recorded.
TAs (91%) showed strong reactivity and full-thickness staining with . TSAs showed full-thickness and weak to intermediate reactivity (79%) and ECF with accentuation was exclusively seen in TSA. SSL showed weak reactivity confined to the basal crypt region (100%). SSL with dysplasia also showed weak or intermediate (100%) reactivity, but the reactivity pattern was full thickness (88%). expression parallels expression (Pearson coefficient=0.63) regarding signal intensity for the examined polyp groups.
Qualitative and quantitative differences in and expression assist in the diagnosis of SSL with dysplasia.
WNT信号通路失调通常是结直肠肿瘤形成的关键早期因素，尤其是染色体不稳定通路。我们使用两种WNT报告基因，即[报告基因名称1]和[报告基因名称2]，旨在评估这些息肉是否表现出可预测的表达模式，以及这些模式是否具有诊断价值。
我们评估了23个腺瘤（TA）、23个无蒂锯齿状病变（SSL）、14个伴有发育异常的SSL以及38个传统锯齿状腺瘤（TSA）。对[报告基因名称1]和[报告基因名称2]进行了显色原位杂交染色（ISH）。反应性被定义为强、中或弱。隐窝上三分之一的反应性被定义为全层染色。记录异位隐窝（ECF）内的强化情况。
TA（91%）显示出对[报告基因名称1]的强反应性和全层染色。TSA显示出全层且对[报告基因名称1]的弱至中等反应性（79%），且仅在TSA中观察到伴有[报告基因名称1]强化的ECF。SSL显示出局限于隐窝底部区域的对[报告基因名称1]的弱反应性（100%）。伴有发育异常的SSL也显示出对[报告基因名称1]的弱或中等反应性（100%），但反应模式为全层（88%）。对于所检查的息肉组，在信号强度方面，[报告基因名称2]的表达与[报告基因名称1]的表达平行（皮尔逊系数 = 0.63）。
[报告基因名称1]和[报告基因名称2]表达的定性和定量差异有助于伴有发育异常的SSL的诊断。
