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鉴定转运体相关蛋白δ作为人类结肠癌细胞中的一种癌基因。

Identification of Translocon-associated Protein Delta as An Oncogene in Human Colorectal Cancer Cells.

作者信息

Amarakoon Darshika, Lee Wu-Joo, Peng Jing, Lee Seong-Ho

机构信息

Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, USA.

出版信息

J Cancer Prev. 2024 Dec 30;29(4):175-184. doi: 10.15430/JCP.24.014.

DOI:10.15430/JCP.24.014
PMID:39790222
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11706732/
Abstract

Identifying the roles of genes in cancer is critical in discovering potential genetic therapies for cancer care. Translocon-associated protein delta (TRAPδ), also known as signal sequence receptor 4 (SSR4), is a constituent unit in the TRAP/SSR complex that resides in the endoplasmic reticulum and plays a key role in transporting newly synthesized proteins into the endoplasmic reticulumn. However, its biological role in disease development remains unknown to date. This is the first study to identify the role of TRAPδ/SSR4 in colorectal cancer cells in vitro. Upon successful transient knockdown of , we observed significant reduction of cell viability in all colorectal cancer cell lines tested. Both HCT 116 and SW480 cell lines were significantly arrested at S and G1 phases, while DLD-1 cells were significantly apoptotic. Moreover, stable knockdown HCT 116 and SW480 cells showed significantly lower viability, anchorage-independent growth, and increased S and G1 phase arrests. Overall, we conclude is a potential oncogene in human colorectal cancer cells.

摘要

确定基因在癌症中的作用对于发现潜在的癌症基因治疗方法至关重要。转位子相关蛋白δ（TRAPδ），也称为信号序列受体4（SSR4），是TRAP/SSR复合物的一个组成单位，该复合物位于内质网中，在将新合成的蛋白质转运到内质网中起关键作用。然而，其在疾病发展中的生物学作用至今仍不清楚。这是第一项在体外鉴定TRAPδ/SSR4在结肠直肠癌细胞中作用的研究。在成功短暂敲低后，我们观察到所有测试的结肠直肠癌细胞系的细胞活力显著降低。HCT 116和SW480细胞系均在S期和G1期显著停滞，而DLD-1细胞则显著凋亡。此外，稳定敲低HCT 116和SW480细胞显示出显著较低的活力、非锚定依赖性生长以及S期和G1期停滞增加。总体而言，我们得出结论， 是人类结肠直肠癌细胞中的一个潜在癌基因。 （注：原文中“Upon successful transient knockdown of ”和“Overall, we conclude is a potential oncogene in human colorectal cancer cells.”这两处有缺失信息，翻译时保留了原文的不完整性。）

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/52bc35f41ef0/jcp-29-4-175-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/d6c50d7af1d1/jcp-29-4-175-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/52bc35f41ef0/jcp-29-4-175-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/d6c50d7af1d1/jcp-29-4-175-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/c76e356ba4f9/jcp-29-4-175-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/b662945d1f80/jcp-29-4-175-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b289/11706732/aeb7405dd2e7/jcp-29-4-175-f4.jpg
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