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"SAVE"-Shock-Assisted Viable Extraction: A Minimally Manipulative Method of Processing Regenerative Cells for Clinical Use.

作者信息

Del Vecchio Daniel, Vranis Neil, Alevrogianni Korina, Theodorou Spero

出版信息

Aesthet Surg J Open Forum. 2024 Nov 19;7:ojae112. doi: 10.1093/asjof/ojae112. eCollection 2025.


DOI:10.1093/asjof/ojae112
PMID:39790718
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11712265/
Abstract

BACKGROUND: Extraction of adipose-derived stem cells (ASCs) from the stromal vascular fraction (SVF) has gained significant attention lately in the realm of regenerative medicine. However, finding highly efficient methods of extraction that also comply with the US regulations has prevented widespread clinical use. OBJECTIVES: The objective of this study was to evaluate a novel ASC extraction device to quantify viable ASC extraction and processing efficiency. METHODS: SVF extracted from abdominal fat samples and processed using a novel shock-assisted viable extraction (SAVE) device was tested for stem-cell count and viability. Additionally, time required for processing was recorded. RESULTS: Twelve adipose samples were utilized for this study. After a mean time of 3 min, cell count yield ranged of 47,400 to 189,400 of viable regenerative cells per cc, with an average of 122,464 viable regenerative cells per cc. CONCLUSIONS: SAVE is a novel fat-processing technique with high stem-cell extraction that shows promise from a regulatory, yield, time efficiency, and cost perspective.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/a0a84cd13bfc/ojae112f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/100dc1d1a479/ojae112f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/7006a7cb0133/ojae112f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/235aed83c88d/ojae112f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/a0a84cd13bfc/ojae112f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/100dc1d1a479/ojae112f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/7006a7cb0133/ojae112f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/235aed83c88d/ojae112f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eda3/11712265/a0a84cd13bfc/ojae112f4.jpg

相似文献

[1]
"SAVE"-Shock-Assisted Viable Extraction: A Minimally Manipulative Method of Processing Regenerative Cells for Clinical Use.

Aesthet Surg J Open Forum. 2024-11-19

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[6]
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[7]
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本文引用的文献

[1]
Nanofat: A therapeutic paradigm in regenerative medicine.

World J Stem Cells. 2021-11-26

[2]
Regenerative and stem cell-based techniques for facial rejuvenation.

Exp Biol Med (Maywood). 2021-8

[3]
Efficacy and safety of stem cell therapy for the early-stage osteonecrosis of femoral head: a systematic review and meta-analysis of randomized controlled trials.

Stem Cell Res Ther. 2020-10-19

[4]
Subcutaneous Injections of Nanofat Adipose-derived Stem Cell Grafting in Facial Rejuvenation.

Plast Reconstr Surg Glob Open. 2020-1-20

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Clin Plast Surg. 2019-10-21

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JAMA Facial Plast Surg. 2016-9-1

[7]
Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue.

Springerplus. 2015-11-23

[8]
A novel and effective strategy for the isolation of adipose-derived stem cells: minimally manipulated adipose-derived stem cells for more rapid and safe stem cell therapy.

Plast Reconstr Surg. 2014-6

[9]
The Celution System: Automated Processing of Adipose-Derived Regenerative Cells in a Functionally Closed System.

Adv Wound Care (New Rochelle). 2014-1-1

[10]
Age influence on stromal vascular fraction cell yield obtained from human lipoaspirates.

Cytotherapy. 2014-8

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