"SAVE"-Shock-Assisted Viable Extraction: A Minimally Manipulative Method of Processing Regenerative Cells for Clinical Use.

BACKGROUND

Extraction of adipose-derived stem cells (ASCs) from the stromal vascular fraction (SVF) has gained significant attention lately in the realm of regenerative medicine. However, finding highly efficient methods of extraction that also comply with the US regulations has prevented widespread clinical use.

OBJECTIVES

The objective of this study was to evaluate a novel ASC extraction device to quantify viable ASC extraction and processing efficiency.

METHODS

SVF extracted from abdominal fat samples and processed using a novel shock-assisted viable extraction (SAVE) device was tested for stem-cell count and viability. Additionally, time required for processing was recorded.

RESULTS

Twelve adipose samples were utilized for this study. After a mean time of 3 min, cell count yield ranged of 47,400 to 189,400 of viable regenerative cells per cc, with an average of 122,464 viable regenerative cells per cc.

CONCLUSIONS

SAVE is a novel fat-processing technique with high stem-cell extraction that shows promise from a regulatory, yield, time efficiency, and cost perspective.