Johansen Torild, Hanebrekke Tanja, Besnier Francois, Westgaard Jon Ivar, Bruvold Ingrid Marie, Dahle Geir, Unneland Laila, Torp Christensen Helle, Saha Atal
Institute of Marine Research (IMR) Tromsø Norway.
Institute of Marine Research (IMR) Bergen Norway.
Ecol Evol. 2025 Jan 8;15(1):e70767. doi: 10.1002/ece3.70767. eCollection 2025 Jan.
The genus in the North Atlantic comprises of long lived deep-waters species that have been extensively fished upon, and many stocks are severely depleted across the Atlantic. This is particularly evident for the species . In recent papers, cryptic species have been indicated within this genus and molecular markers are therefore needed to provide identification for the species, including the cryptic species as a basis for advice regarding management and rebuilding of the stocks. A suite of 2800 Single Nucleotide Polymorphism (SNP) markers were identified from ddRAD sequencing data, of which 56 SNPs were organized in two multiplex reactions and tested on 191 spp. from different sampling locations from Norway and Greenland. Good-quality amplification products were successfully obtained from 49 SNP markers for species ID, and 3 TaqMan probes were designed to successfully assign , , and the two cryptic species types A and B. A total 47 SNPs were biallelic, with averaged per locus ranging between 0.053 and 0.50. This SNP-based method establishes a foundation for genetically identifying the Northeast Atlantic species. The findings presented should be followed by an effort to look for morphological characters to recognize the cryptic species on site. In general, the SNP markers are a proper tool for monitoring the distribution of the species from a management perspective.
北大西洋的[该属名称]属包括寿命长的深水物种，这些物种已被大量捕捞，整个大西洋的许多种群都严重枯竭。这在[物种名称]物种中尤为明显。在最近的论文中，已指出该属内存在隐存种，因此需要分子标记来鉴定[该属名称]物种，包括隐存种，作为有关种群管理和恢复建议的基础。从ddRAD测序数据中鉴定出一组2800个单核苷酸多态性（SNP）标记，其中56个SNP被组织成两个多重反应，并在来自挪威和格陵兰不同采样地点的191个[该属名称]物种上进行了测试。成功从49个SNP标记获得了用于物种鉴定的高质量扩增产物，并设计了3个TaqMan探针来成功区分[物种名称1]、[物种名称2]以及两种隐存种A和B型。总共有47个SNP是双等位基因的，每个位点的平均[具体数值]在0.053至0.50之间。这种基于SNP的方法为东北大西洋[该属名称]物种的基因鉴定奠定了基础。在本研究结果之后，应努力寻找形态特征以便现场识别[该属名称]隐存种。一般来说，从管理角度来看，SNP标记是监测该物种分布的合适工具。
