Weese J L, Gilbertson E M, Syrjala S E, Whitney P D, Starling J R
Dis Colon Rectum. 1985 Apr;28(4):217-21. doi: 10.1007/BF02554033.
Intra-abdominal operations result in profound immunodepression during a period when tumor cells are released into the systemic and portal circulations. This combination may augment tumor metastases. The authors have developed a model in which rat colon carcinoma cells transplanted into the portal vein consistently induce hepatic metastases by four weeks, and death within nine weeks. Additionally, the authors have shown that perioperative treatment with levamisole significantly reduces the incidence of metastases. This study tested whether maleic anhydride-divinyl ether-2 (MVE-2), a known immunostimulant, would produce similar effects. Rats pretreated with MVE-2 the day before and day of tumor implantation developed fewer metastases (34 percent of animals treated with MVE-2, compared with 5 percent of animals not treated with MVE-2 had less than or equal to two liver metastases). Eighteen percent of MVE-2-treated rats developed no hepatic metastases. Comparison of median liver weights between the MVE-2-treated group and the nontreated, tumor-bearing group was significant (P = 0.03) and the MVE-2-treated animals had significantly prolonged survival (P = 0.04). The authors conclude that the perioperative period is critical for the implantation and growth of metastases and that perioperative immunostimulation may be a factor in decreasing the incidence of metastases. This model may have relevance to the adjuvant treatment of human colonic cancer.
腹部手术会在肿瘤细胞释放进入体循环和门静脉循环的时期导致严重的免疫抑制。这种情况可能会增加肿瘤转移。作者建立了一个模型，将大鼠结肠癌细胞移植到门静脉中，4周时会持续诱发肝转移，9周内导致死亡。此外，作者还表明，围手术期使用左旋咪唑治疗可显著降低转移发生率。本研究测试了已知的免疫刺激剂马来酸酐-二乙烯基醚-2（MVE-2）是否会产生类似效果。在肿瘤植入前一天和植入当天用MVE-2预处理的大鼠发生的转移较少（接受MVE-2治疗的动物中有34%发生转移，而未接受MVE-2治疗的动物中有5%发生了小于或等于两处肝转移）。18%接受MVE-2治疗的大鼠未发生肝转移。MVE-2治疗组与未治疗的荷瘤组之间的肝脏中位数重量比较有显著差异（P = 0.03），且接受MVE-2治疗的动物存活时间显著延长（P = 0.04）。作者得出结论，围手术期对于转移瘤的植入和生长至关重要，围手术期免疫刺激可能是降低转移发生率的一个因素。该模型可能与人类结肠癌的辅助治疗有关。
