带尾分子信标探针：一种检测结构化DNA和RNA分析物的方法。

Tailed molecular beacon probes: an approach for the detection of structured DNA and RNA analytes.

作者信息

Mueller Brittany L, Molden Tatiana A, Hammock Jordan, Kolpashchikov Dmitry M

机构信息

Chemistry Department, University of Central Florida, Orlando, Florida 32816, USA.

National Center for Forensic Science University of Central Florida, Orlando, Florida 32816, USA.

出版信息

Chem Commun (Camb). 2025 Jan 28;61(10):2095-2098. doi: 10.1039/d4cc05984k.

DOI:10.1039/d4cc05984k

PMID:39792394

Abstract

Molecular beacon (MB) probes have been extensively used for nucleic acid analysis. However, MB probes fail to hybridize with folded DNA or RNA. Here, we demonstrate that MB probes equipped with extra sequences complementary to the analyte, named 'tail', can increase the signal-to-background ratio by ∼40-fold and hybridization rates by ∼800-fold compared to conventional MB probes. Tailed MB probes can be used as mismatched-tolerant alternatives to traditional hairpin probes for fast assays.

摘要

分子信标（MB）探针已被广泛用于核酸分析。然而，MB探针无法与折叠的DNA或RNA杂交。在此，我们证明，配备了与分析物互补的额外序列（称为“尾巴”）的MB探针，与传统MB探针相比，可将信噪比提高约40倍，杂交率提高约800倍。带尾MB探针可作为传统发夹探针的错配耐受替代品，用于快速检测。