Chen Kuanchang, Dores-Sousa José Luís, Fontana Alberto, Grosanu Cristina, McAllister Heather M, Bai Guoyun, Bartkowiak Karolina, Cañellas Santiago, Corens David, De Groot Alex, Nevarez Juan, Serrano Marta, Raeymaekers Kristien, Rodriguez Raquel, Van Eynde Lars, Zhou Ronghui, Shi Zhicai
Chemistry Capabilities, Analytical & Purification, Global Discovery Chemistry. Janssen Research & Development, a Division of Janssen Pharmaceuticals, Johnson & Johnson company, 1400 McKean Rd. Spring House PA 19477, USA.
Chemistry Capabilities, Analytical & Purification, Global Discovery Chemistry. Janssen Research & Development, a Division of Janssen Pharmaceutica NV, Johnson & Johnson company, Turnhoutseweg 30, Beerse B-2340, Belgium.
J Chromatogr A. 2025 Feb 8;1742:465648. doi: 10.1016/j.chroma.2024.465648. Epub 2024 Dec 31.
In recent years, the need to accelerate drug discovery processes in the pharmaceutical industry has revived the interest of implementing automated workflows, allowing the simultaneous processing of multiple samples on global processes that are referred as High-Throughput Purification (HTP). In this work, SAPIO Laboratory Information Management System (SAPIO LIMS) has been customized at the HTP laboratories of Janssen R&D to accommodate the needs of global purification groups on several automated HTP workflows, integrating Analytical Studio™ data processing tool on multiple steps. Herein we describe the workflow details from crude analysis via RP-LC-MS or SFC-MS systems to sample redissolution and delivery to Compound Logistics (CL) in tubes ready for assay plate preparation. This includes robotic platforms to streamline sample handling and automation tools to facilitate chromatographic and Nuclear Magnetic Resonance (NMR) analyses. The combination of Analytical Studio and the SAPIO LIMS has increased the productivity of Janssen HTP teams since 2020, having a big impact on reducing the Design-Make-Test-Analyze (DMTA) cycles. To the best of our knowledge, this is the first time that a complete HTP workflow with a LIMS is being reported, including RP-HPLC and/or SFC, High-Throughput Nuclear Magnetic Resonance (HT-NMR), redissolution and submission as DMSO solutions of registered compounds to CL, ready for biological assay distribution.
近年来，制药行业加快药物研发进程的需求重新唤起了人们对实施自动化工作流程的兴趣，这使得在被称为高通量纯化（HTP）的全球流程中能够同时处理多个样品。在这项工作中，赛匹欧实验室信息管理系统（SAPIO LIMS）已在杨森研发公司的HTP实验室进行了定制，以满足全球纯化团队在多个自动化HTP工作流程方面的需求，并在多个步骤中集成了Analytical Studio™数据处理工具。在此，我们描述了从通过反相液相色谱 - 质谱联用（RP-LC-MS）或超临界流体色谱 - 质谱联用（SFC-MS）系统进行粗分析到样品重新溶解并以准备好用于制备测定板的试管形式交付给化合物物流（CL）的工作流程细节。这包括用于简化样品处理的机器人平台以及便于进行色谱分析和核磁共振（NMR）分析的自动化工具。自2020年以来，Analytical Studio和SAPIO LIMS的结合提高了杨森HTP团队的生产力，对减少设计 - 制造 - 测试 - 分析（DMTA）周期产生了重大影响。据我们所知，这是首次报道一个完整的带有实验室信息管理系统的HTP工作流程，包括反相高效液相色谱（RP-HPLC）和/或超临界流体色谱（SFC）、高通量核磁共振（HT-NMR）、重新溶解以及将已注册化合物的二甲基亚砜（DMSO）溶液提交给CL，以准备进行生物测定分发。
