降钙素的镇痛活性与中枢5-羟色胺能系统

The analgesic activity of calcitonin and the central serotonergic system.

作者信息

Clementi G, Amico-Roxas M, Rapisarda E, Caruso A, Prato A, Trombadore S, Priolo G, Scapagnini U

出版信息

Eur J Pharmacol. 1985 Jan 15;108(1):71-5. doi: 10.1016/0014-2999(85)90284-5.

Abstract

The effect of peripherally administered cyproheptadine or reserpine and the administration of 5,7-dihydroxytryptamine (5,7-DHT) in the nucleus raphe dorsalis on the analgesic activity of salmon calcitonin (sCT) injected into the lateral ventricle were investigated in male rats. Cyproheptadine or reserpine, given respectively 30 min or 24 h before the peptide, completely abolished the analgesic activity at all the times studied. However, when reserpine was given before the peptide it increased the effect of sCT at 30 (P less than 0.01), 60 (P less than 0.001), 120 (P less than 0.01) and 180 (P less than 0.01) min. 5,7-DHT injected in the nucleus raphe dorsalis 15 days before the peptide led to complete abolition of the analgesic activity. If neurotoxin was injected 4 days before sCT, the effect of the peptide was significant (P less than 0.05) only at 60 min. The results obtained confirm that the analgesic activity of sCT may involve central serotonergic pathway(s), and that the midbrain raphe nuclei 5-HT content is an important focus for this activity.

摘要

在雄性大鼠中，研究了外周给予赛庚啶或利血平以及在中缝背核注射5,7 - 二羟色胺（5,7 - DHT）对侧脑室注射鲑鱼降钙素（sCT）镇痛活性的影响。在给予肽类药物前30分钟或24小时分别给予赛庚啶或利血平，在所有研究时间点均完全消除了镇痛活性。然而，当在肽类药物之前给予利血平时，它在30（P < 0.01）、60（P < 0.001）、120（P < 0.01）和180（P < 0.01）分钟时增强了sCT的作用。在给予肽类药物前15天在中缝背核注射5,7 - DHT导致镇痛活性完全消除。如果在sCT前4天注射神经毒素，肽类药物的作用仅在60分钟时显著（P < 0.05）。获得的结果证实，sCT的镇痛活性可能涉及中枢5 - 羟色胺能通路，并且中脑缝际核5 - HT含量是该活性的重要作用点。