脂质体可诱导人培养细胞中的染色体畸变。

Liposomes induce chromosome aberrations in human cultured cells.

作者信息

Nuzzo F, Sala F, Biondi O, Casati A, Cestaro B, de Carli L

出版信息

Exp Cell Res. 1985 Apr;157(2):397-408. doi: 10.1016/0014-4827(85)90125-9.

Abstract

The genotoxic effect of multilamellar lipid vesicles (MLV) was analysed on cultured heteroploid and diploid human cells. Dose-dependent reduction of cell survival and mitotic rate as well as induction of chromosome aberrations were observed. Chromatid and chromosome breaks and chromatid exchanges were found in 24-h culture after liposome treatment, whereas chromosome rearrangements were prevalent at 48 h. Neutral (PC/Chol) and positive (PC/SA) MLV showed a greater damage than negative (PS/PC; PS) MLV. Fibroblasts were the most sensitive cell type. In the case of PC/Chol MLV vesicles, control experiments with PC and Chol of controlled purity ruled out the possibility that the observed chromosome aberrations were caused by toxic oxidation products present in commercial preparations.

摘要

分析了多层层状脂质体（MLV）对培养的异倍体和二倍体人类细胞的遗传毒性作用。观察到细胞存活率和有丝分裂率呈剂量依赖性降低以及染色体畸变的诱导。脂质体处理后24小时培养物中发现了染色单体和染色体断裂以及染色单体交换，而48小时时染色体重排较为普遍。中性（PC/胆固醇）和阳性（PC/SA）MLV比阴性（PS/PC；PS）MLV造成的损伤更大。成纤维细胞是最敏感的细胞类型。对于PC/胆固醇MLV囊泡，用纯度受控的PC和胆固醇进行的对照实验排除了观察到的染色体畸变是由市售制剂中存在的有毒氧化产物引起的可能性。