Stewart-DeHaan P J, Creighton M O, Larsen L E, Jacobi J H, Sanwal M, Baskerville J C, Trevithick J R
Exp Eye Res. 1985 Jan;40(1):1-13. doi: 10.1016/0014-4835(85)90103-4.
Rat ocular lenses exposed to pulsed microwave irradiation were maintained at constant temperature by circulating phosphate buffered saline in a thermostatically-controlled chamber. Irradiations with pulsed radiation (10 musec, 24 kW pulses) of 918 MHz were done at several different specific absorption rates (SAR) for durations up to 1 hr in order to explore a possible reciprocal relationship. The extent of damage was measured by the maximum depth of granular degeneration in the equatorial region of lenses fixed immediately after irradiation. The parameters of the pulses were increased to 20 musec and 48 kW to explore the variation in the biological effects and threshold with respect to average power, as well as pulse parameters (pulse width, peak power and energy per pulse). A total of 47 lenses were used in 3 X 4 factorial experimental design to explore effects observed at different average powers and durations (6, 20 and 60 min). The results were statistically analyzed by ANOVA and multiple regression analysis with logarithmic transformation. The results are summarized as follows. This data showed clear trends towards increasing depth of granular degeneration with increasing duration of exposure and dose rate. There was considerable evidence to confirm such reciprocity suggesting that total dose is an important parameter. A model postulating reciprocity was shown to explain observed variation in depth of damage as well as one allowing for separate effects of duration and dose rate. Lens fibre cell effects were detected by scanning electron microscopy after 6 min irradiation at the SAR values of 40 and 20 mW g-1. Light microscopic evidence of lens fiber cell damage can be detected at an SAR of 10 mW g-1 after a 1 hr exposure.
将暴露于脉冲微波辐射下的大鼠眼晶状体置于恒温箱中，通过循环磷酸盐缓冲盐水来维持恒定温度。使用918 MHz的脉冲辐射（10微秒，24千瓦脉冲），在几个不同的比吸收率（SAR）下进行长达1小时的照射，以探索可能的反比关系。在照射后立即固定的晶状体赤道区域，通过颗粒变性的最大深度来测量损伤程度。将脉冲参数增加到20微秒和48千瓦，以探索生物效应和阈值相对于平均功率以及脉冲参数（脉冲宽度、峰值功率和每个脉冲的能量）的变化。在3×4析因实验设计中总共使用了47个晶状体，以探索在不同平均功率和持续时间（6、20和60分钟）下观察到的效应。通过方差分析和对数变换后的多元回归分析对结果进行统计分析。结果总结如下。这些数据显示出随着暴露持续时间和剂量率的增加，颗粒变性深度增加的明显趋势。有大量证据证实这种反比关系，表明总剂量是一个重要参数。一个假设反比关系的模型被证明可以解释观察到的损伤深度变化，以及一个考虑持续时间和剂量率单独效应的模型。在40和20 mW g-1的SAR值下照射6分钟后，通过扫描电子显微镜检测到晶状体纤维细胞效应。在10 mW g-1的SAR下暴露1小时后，可以通过光学显微镜检测到晶状体纤维细胞损伤的证据。
