前列环素的高效液相色谱测定法

High-performance liquid chromatographic determination of prostacyclin.

作者信息

Hill G T

出版信息

J Chromatogr. 1979 Sep 1;176(3):407-12. doi: 10.1016/s0021-9673(00)89459-5.

Abstract

A high-performance liquid chromatographic method for the analysis of prostacyclin using a laboratory prepared reversed-phase column packing is described. A relative standard deviation of less than 1% was obtained for ten replicate injections. The system resolves prostacyclin from its hydrolysis product, 6-oxo-prostaglandin F1 alpha and from other prostaglandins present as impurities. These can be estimated to levels of approximately 0.5%. The separation of other unrelated prostaglandins by this method is briefly reported.

摘要

本文描述了一种使用实验室制备的反相柱填料分析前列环素的高效液相色谱法。十次重复进样的相对标准偏差小于1%。该系统可将前列环素与其水解产物6-氧代-前列腺素F1α以及作为杂质存在的其他前列腺素分离。这些杂质的检测限约为0.5%。本文还简要报道了用该方法分离其他不相关前列腺素的情况。