晶状体上皮与放射性白内障。V. 酸性磷酸酶及子午线行核碎裂的观察

Lens epithelium and radiation cataract. V. Observations on acid phosphatase and meridional row nuclear fragmentation.

作者信息

Broglio T M, Worgul B V

出版信息

Exp Eye Res. 1985 Feb;40(2):263-71. doi: 10.1016/0014-4835(85)90011-9.

Abstract

The influence of X-radiation on acid phosphatase activity in differentiating meridional row cells of rat lens epithelia was examined by ultrastructural cytochemistry. X-ray doses of 10-12 Gy (1000-1200 rads) produced clearly observable nuclear and cytoplasmic damage at 13-19 hr postirradiation. In those cells, acid phosphatase reaction product was associated with much of the electron dense material of fragmented nuclei and various parts of the cytoplasm. In addition, many irradiated cells without observable damage were positive for reaction product. The presence of acid phosphatase activity in these otherwise normal-appearing cells is suggestive of more subtle radiation damage than that observed in the more severely damaged cells.

摘要

通过超微结构细胞化学方法，研究了X射线辐射对大鼠晶状体上皮细胞分化中的子午线细胞酸性磷酸酶活性的影响。10 - 12 Gy（1000 - 1200拉德）的X射线剂量在照射后13 - 19小时产生了明显可观察到的细胞核和细胞质损伤。在这些细胞中，酸性磷酸酶反应产物与破碎细胞核的许多电子致密物质以及细胞质的各个部分相关。此外，许多没有可观察到损伤的受辐照细胞对反应产物呈阳性。这些外观正常的细胞中酸性磷酸酶活性的存在表明，与在损伤更严重的细胞中观察到的损伤相比，存在更细微的辐射损伤。