成年火鸡晶状体中δ-晶状体蛋白的表征与结晶：与成年鹌鹑和雏鸡的δ-晶状体蛋白的比较

Characterization and crystallization of delta-crystallin from the eye lens of adult turkey: comparison with delta-crystallin from adult quail and young chick.

作者信息

Narebor E M, Slingsby C

出版信息

Exp Eye Res. 1985 Feb;40(2):273-83. doi: 10.1016/0014-4835(85)90012-0.

Abstract

delta-Crystallin, purified from either adult turkey or quail lenses, and characterized by amino acid composition and circular dichroism spectroscopy, is shown to be similar to delta-crystallin purified from 3-day-old chick lenses. Crystallization of turkey delta-crystallin is reported along with the characterization of the crystal unit cell by X-ray diffraction techniques. Variation in unit cell parameters is observed and is correlated with hydration.

摘要

从成年火鸡或鹌鹑晶状体中纯化得到的δ-晶状体蛋白，通过氨基酸组成和圆二色光谱进行表征，结果表明其与从3日龄雏鸡晶状体中纯化得到的δ-晶状体蛋白相似。报道了火鸡δ-晶状体蛋白的结晶情况以及通过X射线衍射技术对晶体晶胞的表征。观察到晶胞参数的变化，并将其与水合作用相关联。