Yang Xiyan, Nie Jingkun, Zhang Yaxuan, Wang Suqing, Zhu Xiaoping, Li Zhili, Zhao Yunxiang, Shang Xiuguo
School of Life Science and Engineering, Foshan University, Foshan 528000, China.
School of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Animals (Basel). 2024 Dec 26;15(1):26. doi: 10.3390/ani15010026.
Semen quality and persistence are critical for evaluating the usability of individual boars in AI, a standard practice in pig breeding. We conducted GWASs on various semen traits of Duroc boars, including MOT, DEN, ABN, MMP, AIR, and ROS levels. These traits were assessed using FCM and CASA. A total of 1183 Duroc boars were genotyped using the GeneSeek GGP Porcine 50 K SNP BeadChip. The GWAS was performed using three different models: GLM, MLM, and FarmCPU. Additionally, trait heritability was estimated using single- and multiple-trait PBLUP models, yielding 0.19, 0.29, 0.13, 0.18, 0.11, and 0.14 heritability for MOT, DEN, ABN, MMP, AIR, and ROS, respectively. All semen traits exhibited low heritability except ABN, which demonstrated medium heritability. Nine candidate genes (, , , , , , , , and ) were potentially associated with semen traits. These genes are associated with antioxidant and mitochondrial functions in porcine sperm. Our findings provide insight into the genetic architecture of semen traits in Duroc boars, and the identified SNPs and candidate genes may enhance economic outcomes in the pig breeding industry while improving sperm quality through targeted breeding strategies.
精液质量和持久性对于评估杜洛克公猪在人工授精（猪育种中的一种标准做法）中的可用性至关重要。我们对杜洛克公猪的各种精液性状进行了全基因组关联研究（GWAS），包括运动性（MOT）、密度（DEN）、异常率（ABN）、线粒体膜电位（MMP）、凋亡诱导率（AIR）和活性氧（ROS）水平。这些性状使用流式细胞术（FCM）和计算机辅助精子分析（CASA）进行评估。总共1183头杜洛克公猪使用GeneSeek GGP猪50K单核苷酸多态性（SNP）芯片进行基因分型。GWAS使用三种不同模型进行：广义线性模型（GLM）、混合线性模型（MLM）和FarmCPU。此外，使用单性状和多性状最佳线性无偏预测（PBLUP）模型估计性状遗传力，MOT、DEN、ABN、MMP、AIR和ROS的遗传力分别为0.19、0.29、0.13、0.18、0.11和0.14。除ABN表现出中等遗传力外，所有精液性状的遗传力均较低。九个候选基因（……此处原文未完整列出基因名称）可能与精液性状相关。这些基因与猪精子中的抗氧化和线粒体功能有关。我们的研究结果为杜洛克公猪精液性状的遗传结构提供了见解，所鉴定的单核苷酸多态性（SNPs）和候选基因可能会提高猪育种行业的经济效益，同时通过定向育种策略提高精子质量。
