Zhao Chan, Xu Jian, Liu Yao, Xu Peng, Yi Jin, Feng Lin, Miao Yanyan, Zhang Yongping
College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
National Engineering Technology Research Center for Miao Medicine, Guiyang 550025, China.
Molecules. 2024 Dec 25;30(1):29. doi: 10.3390/molecules30010029.
The aim of the present study was to optimize the process parameters for the extraction and purification of total flavonoids from L., in addition to analyzing their chemical composition and evaluating their activity against varicella-zoster virus (VZV) and antioxidant activity. The optimum extraction process was determined using one-way and response surface methods with the following conditions: ethanol concentration of 82.00%, temperature of 90.29 °C, solid-to-liquid ratio of 1:32.78 g/mL, extraction time of 1.5 h, and two extractions with a yield of 14.98 ± 0.11 mg/g. Purification was then carried out using D101 macroporous resin to obtain a flavonoid purity of 43.00 ± 2.55%, which was 3.38 times higher than that of the crude extract (12.74 ± 1.04%). Further purification was carried out using a 1:9 hybrid column of macroporous resin and polyamide, and the purity of flavonoids was enhanced to 59.02 ± 2.23%, which is 1.37 times higher than that of the macroporous resin purifier (43.00 ± 2.55%) and 4.63 times higher than that of the crude extract (12.74 ± 1.04%). Seventy-nine flavonoids were identified using ultra-performance liquid chromatography-tandem high-resolution mass spectrometry (). In addition, the purified flavonoids showed good anti-VZV and antioxidant activities. Therefore, this study can provide technical support and theoretical basis for the further development and utilization of L. resources.
本研究的目的是优化从[植物名称]中提取和纯化总黄酮的工艺参数,此外还分析其化学成分,并评估其对水痘带状疱疹病毒(VZV)的活性和抗氧化活性。采用单因素法和响应面法确定了最佳提取工艺,条件如下:乙醇浓度82.00%、温度90.29℃、固液比1:32.78 g/mL、提取时间1.5 h,提取两次,得率为14.98±0.11 mg/g。然后使用D101大孔树脂进行纯化,得到的黄酮纯度为43.00±2.55%,比粗提物(12.74±1.04%)高3.38倍。使用大孔树脂和聚酰胺的1:9混合柱进一步纯化,黄酮纯度提高到59.02±2.23%,比大孔树脂纯化产物(43.00±2.55%)高1.37倍,比粗提物(12.74±1.04%)高4.63倍。使用超高效液相色谱-串联高分辨率质谱法鉴定了79种黄酮。此外,纯化后的黄酮表现出良好的抗VZV活性和抗氧化活性。因此,本研究可为[植物名称]资源的进一步开发利用提供技术支持和理论依据。