Voleti Vijaya Kumar, Yusuff Ismail, Abdulkadhar Mohamed Jalaludeen, Al-Sadoon Mohammad Khalid
Crescent School of Pharmacy, B. S. Abdur Rahman Crescent Institute of Science and Technology, GST Road, Vandalur, Chennai 600048, Tamil Nadu, India.
Crescent Global Outreach Mission Research and Development, B. S. Abdur Rahman Crescent Institute of Science and Technology, GST Road, Vandalur, Chennai 600048, Tamil Nadu, India.
Polymers (Basel). 2024 Dec 26;17(1):24. doi: 10.3390/polym17010024.
Nocturnal asthma (NA) is a high-prevalence disease that causes severe respiratory issues, leading to death from early midnight to early morning. In this research, nanoparticulate drug delivery system of methylprednisolone (MP) was developed using chitosan (CH) and pectin (PEC). MP is a synthetic corticosteroid medication widely used for its potent anti-inflammatory activity. Computational simulation study (AI-based blend analysis algorithm) was used to identify a better-mixing polymer with MP. MP nanoparticles were formulated by the ionic gelation method with the combination of CH and PEC. To modify the drug release properties, the formed beads were coated with chitosan succinate (CSSC). The morphological characteristics of the beads were determined by SEM analysis. The X-ray radiographic imaging study was used to observe the intactness of MP beads. Histopathological studies were also carried out to find out the toxicity of the beads in the organs of rats. Pectin and chitosan polymers were selected based on the computational simulation study. SEM analysis revealed that the beads had a spherical shape with a rough outer surface. CSSC-coated beads achieved sustained drug release for up to 24 h. X-ray imaging demonstrated the stability of the beads in acidic pH conditions. In vivo pharmacokinetic studies showed that CSSC-coated beads were more stable in the gastrointestinal tract (GIT) than PEC-CH beads and the pure drug. Histological evaluation confirmed that the beads are nontoxic and safe for use in rats. Based on the findings, it was concluded that CSSC-coated beads of MP exhibited superior release properties, making them suitable for a chronomodulated drug delivery system.
夜间哮喘（NA）是一种高发性疾病，会引发严重的呼吸问题，导致从午夜至清晨期间的死亡。在本研究中，使用壳聚糖（CH）和果胶（PEC）开发了甲基泼尼松龙（MP）的纳米颗粒药物递送系统。MP是一种合成皮质类固醇药物，因其强大的抗炎活性而被广泛使用。采用计算模拟研究（基于人工智能的共混分析算法）来确定与MP混合性更佳的聚合物。通过离子凝胶法将CH和PEC结合制备MP纳米颗粒。为了改变药物释放特性，将形成的微球用琥珀酸壳聚糖（CSSC）包衣。通过扫描电子显微镜（SEM）分析确定微球的形态特征。利用X射线放射成像研究观察MP微球的完整性。还进行了组织病理学研究，以查明微球在大鼠器官中的毒性。基于计算模拟研究选择了果胶和壳聚糖聚合物。SEM分析显示微球呈球形，外表面粗糙。CSSC包衣的微球实现了长达24小时的药物缓释。X射线成像表明微球在酸性pH条件下具有稳定性。体内药代动力学研究表明，CSSC包衣的微球在胃肠道（GIT）中比PEC-CH微球和纯药物更稳定。组织学评估证实微球无毒，可安全用于大鼠。基于这些发现，得出结论：MP的CSSC包衣微球具有优异的释放特性，使其适用于时间调制药物递送系统。
