Lv Ran, Zhang Guoqing, Li Hao, Shi Jianxin, Meng Zhu, Lu Xiaoning, Shan Mingzhu, Yang Jie, Pan Zhangyuan
College of Life Science and Technology, Xinjiang University, Urumqi 830047, China.
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China.
Int J Mol Sci. 2024 Dec 25;26(1):79. doi: 10.3390/ijms26010079.
The growth and development of horns are primarily controlled by the skin. The gene is crucial for epidermal barrier function and may have a significant impact on horn growth. The purpose of this study was to investigate the expression of across different sheep breeds and tissues by utilizing RNA sequencing. Additionally, potential functional sites were identified in conjunction with whole genome sequencing. Our findings revealed that was highly expressed in the scurred horn group as opposed to the normal horn group (SHE). expression was also notably high in the skin across several species. Eight loci that may influence horn size were indicated in this study. Through the alignment of the ALOX12B protein sequence from 16 species, 15 amino acid sites were identified specifically expressed in horned animals. In conclusion, this study established a connection between and horn size and identified a series of functional sites that may serve as molecular markers for reducing the presence of horns in Chinese sheep breeds.
角的生长和发育主要受皮肤控制。该基因对表皮屏障功能至关重要，可能对角的生长有重大影响。本研究的目的是通过RNA测序研究该基因在不同绵羊品种和组织中的表达情况。此外，结合全基因组测序确定了潜在的功能位点。我们的研究结果表明，与正常角组（SHE）相比，该基因在有小角组中高表达。该基因在多个物种的皮肤中表达也显著较高。本研究指出了八个可能影响角大小的基因座。通过对16个物种的ALOX12B蛋白序列进行比对，确定了15个在有角动物中特异性表达的氨基酸位点。总之，本研究建立了该基因与角大小之间的联系，并确定了一系列功能位点，这些位点可能作为减少中国绵羊品种角出现的分子标记。
