Innamorati Giorgia, Sanchez-Petidier Marina, Bergafora Giulia, Codazzi Camilla, Palma Valentina, Camera Francesca, Merla Caterina, André Franck M, Pedraza Maria, Moreno Manzano Victoria, Caramazza Laura, Colella Micol, Marracino Paolo, Balucani Marco, Apollonio Francesca, Liberti Micaela, Consales Claudia
PhD Program in Cellular and Molecular Biology, Department of Biology, University of Rome "Tor Vergata", 00133 Rome, Italy.
Division of Biotechnologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.
Int J Mol Sci. 2024 Dec 27;26(1):147. doi: 10.3390/ijms26010147.
In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.
在组织再生领域，干细胞移植是一种很有前景的治疗策略。为了促进它们的植入，需要控制其增殖和分化。多项研究表明，通过施加连续电场刺激可以控制干细胞的命运。本研究旨在表征特定微秒电脉冲刺激（100 µs + 100 µs的双相脉冲，以250 V/cm的强度施加30分钟）对间充质干细胞（MSC）和诱导神经干细胞（iNSC）的细胞增殖增加的影响。从以下几个方面评估了该效果：（i）细胞计数；（ii）细胞周期；（iii）基因表达；（iv）细胞凋亡。结果显示，刺激后24小时，细胞增殖、细胞周期和细胞凋亡均未受影响，但观察到参与这些过程的特定基因表达存在变化。这些结果促使我们在刺激后72小时内研究细胞增殖情况，发现在这个时间点iNSC数量增加。本研究的主要成果是微秒电脉冲可以调节干细胞增殖。
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