Kraevsky Sergey V, Valueva Anastasia A, Ershova Maria O, Shumov Ivan D, Ivanova Irina A, Kanashenko Sergey L, Ryazantsev Ilya A, Ivanov Yuri D, Pleshakova Tatyana O
Institute of Biomedical Chemistry, Pogodinskaya Str., 10, Moscow 119121, Russia.
Int J Mol Sci. 2024 Dec 30;26(1):210. doi: 10.3390/ijms26010210.
Biomacromolecules generally exist and function in aqueous media. Is it possible to estimate the state and properties of molecules in an initial three-dimensional colloidal solution based on the structure properties of biomolecules adsorbed on the two-dimensional surface? Using atomic force microscopy to study nanosized objects requires their immobilization on a surface. Particles undergoing Brownian motion in a solution significantly reduce their velocity near the surface and become completely immobilized upon drying. Using radial distribution function (RDF) methods, it is possible to obtain information about the presence of short-range or long-range order in the arrangement of immobilized colloidal particles. In this work, RDF is applied to immobilized gold nanoparticles (AuNPs) and horseradish peroxidase molecules on mica. It is shown that AuNPs maintain mobility on the mica surface when water is present. Upon immobilization, AuNPs organize into an amorphous structure exhibiting short-range order. Protein molecules are immobilized randomly, and their surface density is well described by the Poisson distribution.
生物大分子通常在水性介质中存在并发挥功能。能否基于吸附在二维表面上的生物分子的结构特性来估计初始三维胶体溶液中分子的状态和性质？使用原子力显微镜研究纳米尺寸的物体需要将它们固定在表面上。溶液中做布朗运动的粒子在靠近表面时速度会显著降低，并在干燥时完全固定。使用径向分布函数（RDF）方法，可以获得有关固定化胶体粒子排列中短程或长程有序存在的信息。在这项工作中，RDF被应用于云母上固定化的金纳米粒子（AuNP）和辣根过氧化物酶分子。结果表明，当有水存在时，AuNP在云母表面保持流动性。固定后，AuNP组织成具有短程有序的无定形结构。蛋白质分子随机固定，其表面密度可用泊松分布很好地描述。
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